Volume 5 Issue 1 2026 – Journal of Plant Biota

Characterization of Aflatoxin-Producing Fungi on Postharvest Quality of Stored Sorghum (Sorghum bicolor L.) Grains in Makurdi, Benue State

Liamngee, K.¹
Raymond, L.T.²
Awua, Y.¹
Shiriki, D. ¹
Fayinminu, A. O.¹

INTRODUCTION Sorghum (Sorghum bicolor (L.) Moench) is among the major cereal crops cultivated worldwide and represents an important indigenous cereal in Africa. In Nigeria, it is considered one of the principal staple cereals, with an estimated annual production of approximately 8 million tonnes. The crop has diverse applications, serving as a source of human food and livestock feed and as a raw material for the preparation of both alcoholic and non-alcoholic traditional beverages [1]. Across tropical and semi-tropical regions, Sorghum contributes substantially to the dietary supply of energy, protein, vitamins, and essential minerals for both human populations and livestock. Its grains are particularly valued as a carbohydrate-rich food ingredient, while the seeds are also utilized in agricultural production. In Nigeria, Sorghum grains are processed into a variety of traditional foods. In southwestern communities, the grain may be milled and processed into pastes and pap, which is commonly consumed as a complementary food for children. Throughout different parts of Africa, Sorghum is incorporated into traditional products including tuwo and molded grain products such as fura. It is also used in the preparation of fermented alcoholic beverages, including burukutu and pito, as well as non-alcoholic beverages such as kunu zaki [2]. Beyond its traditional uses, Sorghum has gained considerable nutritional interest because it is naturally gluten-free and contains several phytochemicals with potential health-promoting properties. Among its bioactive constituents are phenolic compounds such as vanillic acid, gallic acid, and ferulic acid, which have been associated with antioxidant and anti-inflammatory activities [3]. The nutritional profile of Sorghum grains varies considerably depending on cultivar, environmental conditions, and processing practices. Reported ranges include approximately 1.4–26.1% dietary fibre, 54.6–85.2% carbohydrates, 0.9–4.2% ash, 1.3–10.5% fat, and 6.2–14.9% protein [4]. These nutritional attributes make Sorghum an important component of food systems, particularly in regions where cereals constitute a major proportion of the daily diet. However, despite its nutritional and economic importance, maintaining grain quality after harvest remains a major challenge. STATEMENT OF THE PROBLEM Post-harvest deterioration is one of the major factors responsible for losses in cereal production. In many parts of sub-Saharan Africa, inadequate drying, handling, transportation, and storage facilities contribute substantially to post-harvest losses and consequently aggravate existing food-security challenges [5]. Sorghum grains that are harvested with excessive moisture or exposed to unsuitable storage conditions provide favourable conditions for the development of moulds and other spoilage microorganisms [6]. Prolonged exposure to moisture and elevated temperatures can therefore compromise grain quality and increase the likelihood of fungal contamination. Several fungal genera, particularly Aspergillus, Penicillium, and Fusarium, are capable of colonising improperly stored cereal grains and may produce toxic secondary metabolites known as mycotoxins [7]. Species belonging to Aspergillus are associated with the production of aflatoxins, including aflatoxins B1, B2, G1, and G2, whereas Fusarium species may produce important mycotoxins such as fumonisins, trichothecenes, and zearalenone. The occurrence of aflatoxin-producing fungi is not restricted to a single commodity; these organisms have been reported in several major African food crops, including maize, Sorghum, millet, rice, oilseeds, groundnuts, spices, tree nuts, and cassava [8]. The risk of aflatoxin contamination may arise at different stages of crop production and post-harvest management. Since Aspergillus species are commonly associated with soil and plant environments, contamination may begin during crop establishment and subsequently increase as a result of poor harvesting, handling, drying, transportation, processing, and storage practices. Environmental factors, particularly elevated temperature and moisture availability, strongly influence fungal growth and toxin production both before and after harvest [9]. Among the principal aflatoxin-producing fungi are Aspergillus flavus and Aspergillus parasiticus, which can synthesize aflatoxins B1, B2, G1, and G2 [10]. Aflatoxin B1, which is recognized by the International Agency for Research on Cancer (IARC) as a potent naturally occurring carcinogenic compound [11]. Human and animal exposure to contaminated food and feed can therefore present significant food-safety and public-health concerns. Following harvest, inadequate drying, excessive grain moisture, high storage temperatures, and poor storage conditions may create an environment conducive to fungal proliferation and subsequent mycotoxin formation [9]. Benue State is one of Nigeria’s important Sorghum-producing regions. Given the importance of Sorghum as a food and feed resource in the area, the occurrence of fungal contaminants in stored grains warrants systematic investigation. Therefore, assessing the fungal contamination of stored Sorghum grains is important for determining the prevalence of potentially toxigenic fungi and for generating information that can support improved post-harvest handling and storage practices in the region. Significance of the Study The stakeholders likely to benefit from this study include students, researchers, farmers, and consumers. For farmers, it will guide them on adopting better postharvest handling, drying and storage practices to reduce contamination. For researchers, it will provide baseline data for further research in this area of study. For policymakers, it will provide scientific evidence to support policies on food safety, grain quality control and aflatoxin regulation in Nigeria. For students, it will provide relevant research data for academic research projects. Therefore, theaim of the study was to isolate, identify fungi and quantify aflatoxins in Sorghum (Sorghum bicolor L.) grains in some storehouses in Makurdi. MATERIALS AND METHODS Experimental location The study was carried out in the Institute for Agricultural Research, Zaria, Kaduna state and the Botany laboratory of Rev. Fr. Moses Orshio Adasu University, Makurdi, Benue State. Sorghum samples were sent to the Institute for Agricultural Research, Zaria, Kaduna State, for aflatoxin analysis, while Sorghum samples were brought to the Botany laboratory of Rev. Fr. Moses Orshio Adasu University, Makurdi for isolation of aflatoxin-producing fungi. Collection of Sorghum grain samples Sorghum grains were collected from three major storehouses. One each from Wurukum, Northbank and Yaikyo markets in Makurdi Local Government Area of Benue State. The samples were collected from the storehouses using the sampling probe. The sampling probe was inserted horizontally into the bag and withdrawn slowly in order to collect a uniform sample from each bag. Three samples were collected from each storehouse. Approximately 500g samples were purchased from each storehouse and placed separately in clean containers and labelled appropriately. They were transported to the … Read more

The Mysterious Blak Garlic beyond the Vegetable

Jin-Ichi Sasaki

Introduction The Heat Stress Induced Black Garlic was not a new species in the classification but developed from the fresh garlic by treatment under the heat and moisture control circumstances for around a month without any other chemical additives [1, 2]. This manufacturing method was devised by the villager who had seen the diving women’s heavy work to collect shell from the sea bottom. They took a baked raw garlic before diving to get energy. Villager (Mr. Hamano) who saw and decided to devises some simple way for taking of raw garlic directly. After repeated tests, he finally got the protype of the Black Garlic by controlling temperature and humidity in a garlic processing box. Since the natures of the chemical facts and biofunction were not reported, then we initiated the black garlic research. This paper covers those requires and the evaluation of the black garlic in the world was also introduced. Required conditions to develop the black garlic is just control of temperature and humidity in an incubator without any others. This simplicity is a great advantage for expanding of the temperature and black garlic making among the citizen in Japan. One of the points to note is toward the irritative volatile gas released from garlics processing. Concretely it is better to use the outside of house. 5 Additional other biofunctions of the Black Garlic reported [5] Enforcement of anti-oxidant activity Strengthen immune system Bacteria killing potency Suppress of carcinogenesis effect [6]. 6 World Wide Evaluation of The Black Garli biological effects In May 2006, the lack garlic made a debut via the TV and News Paper in Japan with a Title ’Stroger Antitumor potency than that of the raw garlics’ as shown in Photo 1. We submitted these results to the journal, but rejected of Latin nameof the black garlic It took some times to persuade the referees to explain the situation ‘Black Garlic was developed in Japan with no Latin Name). It was as a historical debut of the black garlic. [2] °Encouraging Words from WHO [６] The black garlic produced in the land of the rising of the sun as it has developed elaborated producing technique (H. Hamano, 2004), This food has become world-famous thanks to recent scientist that has research by Japanese researcher J. Sasaki first demonstrated the benefits. It is good for you and regular consume. The black garlic is recommended by WHO. Lately, the tablet and capsule types of the black garlic were prepared for the client much easier to take. Mail from the Wall Street Journal [7] I am a United St　ates journalist, and I write a consumer health column for the Wall Street Journalist I have seen some of　your work and would love to speak with you or ask you questions by mail or both. Laura Johannnss (2016, Jun, 20 Life Health Aches and Claims.) Le mystere de l’ail noir ( Camille Labro) [8] ALICAMENT ll aprait sur la cote sud-est du Japon au debut des anees 2000. C’est la que sa technique de production semble avoir ete finalisee:less tetes deail sont matuurees de quin a vingt jours s un lieu clos a 60-80C, avec un taux d’humidititede 70 90 % La transforzemation spectaculaire des goussesst a tout s implement, t le resullat d’une (d’tres lente) caramelization , connue dsous le nom de reaction de Ma sont . C’est a Aomori dans le nord du Japon qu’il deviant cerebre, grace aux travax du profen esseur Jin-ichi Sasaki, qui demontreses vertus exceptiooelles, notammentent pour combattalttre cetem. [9] Conclusively Consideration The novel vegetable black garlic becomes almost 20years old after born in Japan He now armed with various bio-functions to help the citizen health conditions to fight the disease. I have a great concern how the black garlic effect to s allergy, cancer, brain works, diabetes et al. Furthermore, lately the herb associate function project has launched in the Saudi Arabia to develop new functional substance from the world scientists. References [1] J. Sasaki. Bioreaction of the Cells against the Stress Burden. European Journal of Sciences vol 14 No ( 01) 2547-257, (2026) [2] J. Sasaki, C. Lu, E., Machiya, et al. Processed Black Garlic Extracts Enhanced Anti-Tumor Potency against Mouse tumor. Medicinal and Aromatic　Plant Science and Biotechnology. Global Science Books, 1(2), 278-282, 2007 [3]　J. Sasaki and H. Hamano., Super Food Black Garlic-Breath of Birth., Current Strategies in Biotechnology and Bioresource Technology Vol. 1. 84-91]. 2010 [4] D. Wang ,Y., Feng, J. Sasaki, et al., Black Garlic Extracts Enhanced the Immune System Medical Aromatic Plant Science and Biotechnology Global Science Books 4 [1] 37-40 2010 [5] J. Sasaki. First choice for your health (in Bilingual Report). 2024 [6] 　T, Ihara T, Bepp, K. Shinpo, et al, Effects of the Black Garlic on 1.2-Dimethylhydrazine -induced Premalignant Lesions in Rat Colon. Fujita Gaku- Report, 31(2), 143-127, 2007.in Japanese [7] 　ence.com/to-kn/chopsticks-at-the-ready/kuro-ninnik-black-garlic [8].　Mle magazine du Mondel (17.01.20141a 1225.a jour le .17.01.20144 a 14h39

Antibiotic Resistance Profile of Campylobacter jejuni from Edible Plant Biota in Kpiri-Kpiri Market, Abakaliki Metropolis, Nigeria

Ifeoma P. Ogbonna¹
Jerry O. Orji²
Ikemesit U Peter³
Bright N Abba¹
Kelechi N Ogboji⁴

INTRODUCTION Edible plant biota, encompassing fruits and vegetables, are cornerstone components of a healthy human diet, providing essential nutrients, vitamins, minerals, and dietary fiber [1]. The global shift towards health-conscious eating has increased the demand for minimally processed and ready-to-eat plant-based foods [2,3]. However, this trend has concurrently elevated the risk of foodborne illnesses, as these foods are often consumed raw, bypassing a critical pathogen reduction step (cooking). Among the myriad of potential contaminants, Campylobacter jejuni has emerged as the leading bacterial cause of human gastroenteritis worldwide [4]. Campylobacter jejuni is a Gram-negative, spiral-shaped, microaerophilic bacterium. Clinical manifestations of C. jejuni infection range from self-limiting acute enteritis (diarrhea, abdominal pain, fever) to severe post-infectious sequelae, including Guillain-Barré syndrome, an autoimmune neurological disorder, and reactive arthritis [5]. Globally, Campylobacter species (predominantly C. jejuni) are estimated to cause over 95 million foodborne illnesses and 21,000 deaths annually, with a disproportionately high burden in low- and middle-income countries, including those in Africa [4]. The contamination of edible plant biota with C. jejuni can occur at multiple points along the farm-to-fork continuum, including primary production (via contaminated irrigation water, soil, or untreated animal manure used as fertilizer), post-harvest handling, transportation, storage, and final preparation [6]. The use of untreated poultry litter and animal feces as organic fertilizer is a particularly significant pathway, as it directly introduces enteric pathogens like C. jejuni into the agricultural environment [7,8]. Open-air markets like Kpiri-Kpiri Market in Abakaliki Metropolis present additional contamination risks due to exposure to dust, flies, poor handling practices, and inadequate storage facilities [9]. Compounding the public health challenge is the escalating threat of antimicrobial resistance (AMR). The overuse and misuse of antibiotics in human and, critically, in animal agriculture have selected for resistant bacterial strains [10]. Food-producing animals, especially poultry, are major reservoirs of C. jejuni, and the use of antibiotics (e.g., tetracyclines, fluoroquinolones) for growth promotion or therapy in these animals drives resistance [8, 11]. Subsequently, resistant C. jejuni can be transmitted to humans through the consumption or handling of contaminated plant-based foods [12]. Despite the global significance, there is a paucity of comprehensive data on the burden and AMR profile of C. jejuni specifically isolated from edible plant biota in specific markets within Abakaliki Metropolis, Nigeria. Therefore, this study was designed to isolate and presumptively identify Campylobacter jejuni from raw fruits and vegetables sold in Kpiri-Kpiri Market, Abakaliki Metropolis, determine its prevalence, and characterize its antimicrobial susceptibility profile. 2. MATERIALS AND METHODS 2.1. Study Area and Description This research was carried out at Kpiri-Kpiri Market, situated within the Abakaliki Metropolis in Ebonyi State, Southeastern Nigeria. Abakaliki, which serves as the state capital, lies at coordinates 6.32°N and 8.12°E [13]. As one of the largest traditional open-air markets in the area, Kpiri-Kpiri Market features a daily assortment of fresh produce including various fruits and vegetables sold under prevailing ambient conditions. 2.2. Sample Collection A total of 60 samples comprising 40 vegetables and 20 fruits were aseptically purchased from Kpiri-Kpiri Market. The vegetable samples included cucumber, African pear, garden egg fruit, fresh tomato, fresh pepper, okro, carrot, garden egg leaf, pumpkin leaf, and cabbage (4 samples each). The fruit samples included tigernut, banana, watermelon, guava, and palm fruit (4 samples each). Each sample was collected into sterile sample bags, placed in a cooler box with ice packs (4-6°C), and transported to the laboratory for analysis within one hour of collection. 2.3. Bacterial Enumeration and Isolation For each sample, 25g was homogenized in 225mL of 0.1% peptone water. Serial ten-fold dilutions were prepared, and 0.1mL from appropriate dilutions was plated onto Campylobacter Blood-Free Selective Agar (Thermo Fisher Scientific, USA). Plates were incubated microaerophilically (using GasPak EZ Campy Container Systems, BD) at 42°C for 48 hours. Colonies exhibiting typical Campylobacter morphology (creamy-yellow, moist, irregular) were subcultured for purity. Total viable bacterial counts were expressed as Colony Forming Units per mL (CFU/mL) [14]. 2.4. Phenotypic Identification of Campylobacter jejuni Standard microbiological techniques were employed for the presumptive identification of Campylobacter isolates. Morphological characterization was first carried out via Gram staining to confirm the presence of Gram-negative, spiral, or curved rod-shaped cells. Motility was assessed using the hanging drop method, observing for characteristic darting motility. The oxidase test was performed using 1% tetramethyl-p-phenylenediamine dihydrochloride. For presumptive identification of C. jejuni, the hippurate hydrolysis test was conducted. Isolates that hydrolyzed sodium hippurate (positive test) were presumptively identified as Campylobacter jejuni. 2.5. Antimicrobial Susceptibility Testing Antibiotic susceptibility was determined for all presumptive C. jejuni isolates (n=8) using the Kirby-Bauer disc diffusion method on Mueller-Hinton agar supplemented with 5% defibrinated sheep blood, following CLSI guidelines [15]. The following ten antibiotic discs (Oxoid, UK) were tested: Ceftazidime (30 µg), Cefotaxime (30 µg), Cefoxitin (30 µg), Imipenem (10 µg), Meropenem (10 µg), Ciprofloxacin (5 µg), Gentamicin (15 µg), Nitrofurantoin (25 µg), Erythromycin (15 µg), and Tetracycline (15 µg). Results were interpreted as Susceptible or Resistant using CLSI breakpoints [15]. The Multiple Antibiotic Resistance (MAR) index was calculated for each isolate as a/b, where ‘a’ is the number of antibiotics to which the isolate was resistant and ‘b’ is the total number of antibiotics tested (10) [8, 15]. 2.6. Statistical Analysis Data were analyzed using GraphPad Prism v9.0. Prevalence was expressed as percentages. Descriptive statistics were used to summarize bacterial loads and resistance patterns. 3. RESULTS 3.1. Bacterial Load of Edible Plant Biota Samples from Kpiri-Kpiri Market The total viable bacterial counts on the sampled fruits and vegetables from Kpiri-Kpiri Market ranged from 6.90 × 10⁴ to 1.94 × 10⁵ CFU/mL. Among the vegetables, Pumpkin Leaf had the highest contamination level at 1.94 × 10⁵ CFU/mL, followed by Okro at 1.43 × 10⁵ CFU/mL and African Pear at 1.20 × 10⁵ CFU/mL. Among the fruits, Watermelon showed the highest bacterial load at 1.70 × 10⁵ CFU/mL, followed by Banana at 1.09 × 10⁵ CFU/mL. The mean bacterial load for all samples was 1.14 × 10⁵ CFU/mL. Table 2 presents a comparative analysis of C. jejuni prevalence in fruits from different geographical locations worldwide. Of the 60 samples analyzed from Kpiri-Kpiri Market, Campylobacter species were isolated from 32 samples, giving an overall Campylobacter prevalence of 53.3%. Among these, presumptive Campylobacter jejuni (hippurate hydrolysis positive) was identified in 8 isolates, representing 25.0% of all Campylobacter-positive samples … Read more

Simultaneous Hepatic and Renal Biochemical Toxicity Following Chronic Petroleum Hydrocarbon Exposure in Chickens

Eruotor O. Harrison¹
Chinwebudu M. Melford²

INTRODUCTION Petroleum hydrocarbons are among the most important environmental contaminants generated through crude oil exploration, transportation, refining, industrial discharge, and accidental spills [1]. In oil-producing communities, continuous contamination of soil and water creates long-term exposure risks for humans and animals [2]. Organisms inhabiting polluted environments may absorb hydrocarbons through ingestion of contaminated feed and water, inhalation of volatile compounds, or dermal exposure [3]. Chronic exposure to petroleum hydrocarbons has been linked with toxic effects involving several organ systems, especially the liver and kidneys because of their critical functions in metabolism, detoxification, and excretion of xenobiotics [4]. The liver is highly susceptible to petroleum hydrocarbon toxicity because it is the principal organ responsible for the metabolism of foreign compounds [5]. Petroleum hydrocarbons include aliphatic compounds, aromatic hydrocarbons, cycloalkanes, and polycyclic aromatic hydrocarbons, many of which are capable of generating reactive intermediates during metabolism [6]. These metabolites may induce oxidative stress, lipid peroxidation, mitochondrial dysfunction, and membrane instability within hepatocytes, leading to hepatocellular injury and altered liver function [7]. Such hepatic damage is commonly reflected by elevated serum aminotransferases and alkaline phosphatase activities, together with disturbances in albumin synthesis, total protein concentration, and bilirubin metabolism [8]. The kidneys are also vulnerable to hydrocarbon toxicity because they participate in the elimination of water-soluble metabolites derived from petroleum hydrocarbons [9]. Exposure to these compounds may impair glomerular filtration and tubular function, resulting in elevated serum urea and creatinine concentrations as well as electrolyte imbalance [10]. Persistent renal injury may further contribute to systemic metabolic disturbances and altered physiological homeostasis [11]. Interactions between hepatic and renal dysfunction are increasingly recognized in toxicological and clinical studies. Damage to the liver may increase circulating toxic metabolites capable of aggravating renal injury, while impaired renal clearance may prolong systemic retention of hepatotoxic compounds [12,13]. Despite this relationship, many studies continue to assess hepatic and renal toxicity separately, thereby limiting understanding of the overall systemic effects of petroleum hydrocarbon exposure. Chickens are considered valuable sentinel organisms in environmental toxicology because they are continuously exposed to environmental contaminants through soil, water, and feed interactions [14]. In addition, chickens are economically important food animals, making environmental toxicant exposure a public health concern [15]. Although previous studies have investigated petroleum hydrocarbon toxicity in poultry, information regarding simultaneous hepatic and renal biochemical responses during chronic exposure remains limited. Therefore, this study was designed to evaluate concurrent hepatic and renal biochemical toxicity in chickens chronically exposed to petroleum hydrocarbon contamination. The study assessed liver enzymes, bilirubin fractions, serum proteins, renal biomarkers, and electrolyte profiles, while also examining the influence of exposure duration on toxicological outcomes. MATERIALS AND METHODS Study design This study adopted a comparative experimental design to investigate hepatic and renal biochemical toxicity in chickens exposed to a petroleum hydrocarbon–contaminated environment. Biochemical findings obtained from exposed chickens were compared with those of unexposed control birds. The study also evaluated the influence of exposure duration on the severity of hepatorenal toxicity. Experimental animals and grouping A total of eighteen chickens were used for the study. Twelve chickens were obtained from an environment characterized by chronic petroleum hydrocarbon contamination associated with prolonged hydrocarbon-related activities. Six chickens obtained from a non-contaminated environment served as controls. The exposed chickens were grouped according to exposure duration. Six chickens were evaluated after 6 months of exposure, while another six chickens were evaluated after 12 months of exposure. Control birds were similarly categorized according to age and duration. All chickens were maintained under similar feeding and husbandry conditions throughout the study period to minimize environmental and nutritional variation. Blood sample collection and serum preparation Blood samples were collected aseptically by venipuncture into clean dry tubes without anticoagulant. The samples were allowed to clot at room temperature and were centrifuged at 3000 rpm for 10 minutes to separate serum. Serum samples were transferred into properly labeled containers and stored at −20 °C pending biochemical analysis. All analyses were completed within 72 hours of sample collection. Biochemical analysis Serum AST and ALT activities were determined using the Reitman–Frankel method, while ALP activity was analyzed using the p-nitrophenyl phosphate kinetic method. Serum albumin concentration was measured using the bromocresol green method, and total protein was determined using the Biuret technique. Total and conjugated bilirubin concentrations were estimated using the diazo reaction method. Renal function was assessed by measuring serum urea and creatinine concentrations using the urease–Berthelot and Jaffe alkaline picrate methods, respectively. Electrolyte analysis included sodium and potassium determination by flame photometry, while chloride and bicarbonate concentrations were measured using standard colorimetric procedures. Commercially available diagnostic kits were used for all biochemical analyses according to the manufacturers’ instructions. Absorbance readings were obtained using a visible spectrophotometer (Model S23A, HELMREASINN, China). To ensure analytical reliability, all assays were performed in duplicate, and internal quality control sera were included during each analytical run. Statistical analysis Data were analyzed using IBM SPSS Statistics version 25.0 (IBM Corp., Armonk, NY, USA). Results were expressed as mean ± standard deviation. Comparisons between exposed and control groups were performed using independent-sample t-tests, while one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test was used to assess differences associated with exposure duration. Statistical significance was accepted at p < 0.05. Ethical considerations All experimental procedures involving animals were carried out in accordance with the National Research Council Guide for the Care and Use of Laboratory Animals (8th edition). Measures were implemented to minimize animal discomfort during handling and sample collection. RESULTS Hepatic biochemical alterations The hepatic biochemical parameters of exposed and control chickens are presented in Table 1. Chickens exposed to petroleum hydrocarbon contamination showed significant hepatic dysfunction when compared with controls (p < 0.05) . Serum AST, ALT, and ALP activities were significantly elevated in exposed birds, findings consistent with previous reports of petroleum hydrocarbon–induced hepatocellular injury and membrane destabilization in exposed animals and poultry species [16, 17]. In contrast, serum albumin and total protein concentrations were significantly lower in exposed chickens, suggesting impaired hepatic synthetic function under chronic toxic stress. Total and conjugated bilirubin concentrations … Read more

Evaluation of Acetone, Methanol, and Hexane Extracts from Three Medicinal Plants for the Control of Fusarium Wilt of Potato In Vitro

Perpetual M. Katsriku
Julius Onyango Ochuodho

Introduction Fusarium wilt is a major vascular disease affecting Irish potatoes (Solanum tuberosum L.) worldwide. Fusarium oxysporum (F. oxysporum), a soil-dwelling pathogen, is the primary causative agent. The pathogen infiltrates the root system and commandeers the xylem vessels, resulting in wilting, chlorosis, stunting, and a significant reduction in tuber yield. Controlling this disease is challenging due to the prolonged viability of the pathogen’s chlamydospores in soil and the limited availability of resistant potato varieties [20]. The existing dependence on synthetic fungicides prompts apprehensions regarding environmental toxicity, disease resistance, and human health, hence necessitating the exploration of sustainable, eco-friendly alternatives [6]. Plant-derived extracts may serve as a viable source of antimicrobial agents for the formulation of bio-fungicides. This approach adheres to the principles of integrated pest management (IPM) and the increasing demand for safer agricultural chemicals [10]. Euclea divinorum Hiern (Ebenaceae), Prunus africana (Hook.f.) Kalkman (Rosaceae), and Carissa edulis (Forssk.) Vahl (Apocynaceae) are ethnobotanically important plants in East Africa, historically employed for the treatment of diverse health conditions. This signifies that secondary metabolism is exceptionally resilient. E. divinorum is acknowledged for its antibacterial and antioxidant attributes, ascribed to naphthoquinones and triterpenoids [11]. Comprehensive studies have been undertaken on the bark of P. africana, which encompasses various bioactive compounds, including phytosterols. Nonetheless, limited research has been undertaken regarding its leaves [19]. Nyagumbo propose that C. edulis possesses antibacterial activities and anti-inflammatory advantages [15]. Despite the acknowledged advantages of these plants, there has been insufficient research on their efficacy against phytopathogenic fungi, particularly Fusarium oxysporum in potatoes. The extraction solvent is essential as it modifies the polarity and spectrum of the isolated molecules, hence affecting their bioactivity [13]. Acetone, methanol, and hexane exhibit varying polarities, enabling the extraction of a range of antimicrobial compounds, from non-polar terpenoids in hexane to medium-polarity phenolics in acetone and high-polarity glycosides in methanol. This study evaluated the effectiveness of acetone, methanol, and hexane extracts from the leaves, roots, and stems of Euclea divinorum, Prunus africana, and Carissa edulis against Fusarium oxysporum, the pathogen that causes wilt in Irish potatoes. The results will enhance the scientific validation of these ethnobotanical resources and pinpoint potential lead extracts for the formulation of botanical fungicides. Fusarium wilt, caused primarily by the soil-borne pathogen Fusarium oxysporum (F. oxysporum), is a devastating vascular disease affecting Irish potato (Solanum tuberosum L.) cultivation worldwide [12]. The pathogen invades the root system, colonises the xylem vessels, and leads to wilting, chlorosis, stunting, and significant tuber yield losses. Managing this disease is challenging due to the longevity of the pathogen’s chlamydospores in soil and the limited availability of resistant potato cultivars [20]. Current dependence on synthetic fungicides raises concerns regarding environmental toxicity, pathogen resistance, and human health, driving the search for sustainable, eco-friendly alternatives [6]. Plant-derived botanicals represent a promising reservoir of antimicrobial compounds, offering a potential basis for bio-fungicide development. This approach aligns with integrated pest management (IPM) principles and the growing demand for safer agricultural inputs [10]. Euclea divinorum Hiern (Ebenaceae), Prunus africana (Hook.f.) Kalkman (Rosaceae), and Carissa edulis (Forssk.) Vahl (Apocynaceae) are ethnobotanically significant species in East Africa, traditionally used to treat various ailments, indicative of their rich secondary metabolism. E. divinorum, for instance, is renowned for its antimicrobial and antioxidant properties, attributed to naphthoquinones and triterpenoids [11]. P. africana bark is rich in bioactive compounds like phytosterols and is widely studied; however, its leaves are less explored [19]. C. edulis is reported to possess antimicrobial and anti-inflammatory activities [15]. While these plants have documented uses, their efficacy against phytopathogenic fungi, specifically Fusarium oxysporum affecting potatoes, remains underexplored. The choice of extraction solvent is critical, as it influences the polarity and spectrum of compounds recovered, thereby affecting bioactivity [13]. Acetone, methanol, and hexane offer a range of polarities, facilitating the extraction of diverse antimicrobial compounds, from non-polar terpenoids in hexane to medium-polarity phenolics in acetone and high-polarity glycosides in methanol. Therefore, this study aimed to evaluate the in vitro antifungal activity of leaf, root, and stem bark extracts of Euclea divinorum, Prunus africana, and Carissa edulis, prepared using acetone, methanol, and hexane, against Fusarium oxysporum causing wilt in Irish potato. The findings will contribute to the scientific validation of these ethnobotanical resources and identify potential lead extracts for developing botanical fungicides. Methodology 2.1. Isolation and identification of Fusarium oxysporum Irish Potato plants exhibiting indications of Fusarium wilt were transported to the laboratory in sterile paper bags. The Irish potato plants were individually washed with tap water to eliminate dirt, cut into 1 cm fragments, surface sterilised with 0.1% sodium hypochlorite for three minutes, and rinsed three times alternately with sterile distilled water. The specimens were subsequently infected at five equidistant locations on PDA within a 90 mm Petri dish and cultured for 7 days at room temperature (28 ± 2 °C). Mycelia were sub-cultured onto new PDA, and additional sub-culturing was performed until pure cultures of F. oxysporum were achieved. Slides of 7-day-old mycelia from pure cultures of F. oxysporum were analysed using a compound microscope (Leica DME, Leica Microsystems, Shanghai, China). The identification as F. oxysporum was validated by contrasting their physical and cultural traits with the photographs and descriptions provided by [2]. 2.2. Collection of Plant Samples Leaves and roots of Euclea divinorum Hiern (Ebenaceae) and Carissa edulis (Forssk.) Vahl (Apocynaceae) and the stem barks and leaves of Prunus africana (Hook.f.) Kalkman (Rosaceae) were intentionally harvested from Elgeyo Marakwet County in 2025. The specimen was subsequently transported to the Department of Botany Herbarium for verification and allocation of a voucher specimen number. 2.3. Preparation of Plant Extracts The roots, leaves, and stem barks of the collected plants were washed with tap water and distilled water, then shade-dried for two weeks. The materials were converted into fine powders using a laboratory grinding mill. Subsequently, they were enclosed in airtight bags, tagged, and maintained in darkness until extraction. Approximately 50 grams of each plant powder were measured and extracted with 0.5 litres of ethanol by continuously swirling with a magnetic stirrer for three hours. The extract was subjected to filtration using filter paper and subsequently evaporated to dryness under vacuum conditions. The extract was … Read more

A Comprehensive Review on Argyeria Nervosa

Tushar Chaudhary
Owais Mohd Qureshi
Tarun Parashar

Argyeria nervosa (family: Convolvulaceae), known as elephant creeper or wooly morning glory, grows along river banks, lake margins, and in semi-deciduous forest undergrowth. Its leaves contain quercetin-1-triacontanol, β-sitosterol and quercetin. The plant has been traditionally employed for anti-viral, anti-bacterial, anti-fungal and anti-inflammatory purposes and is claimed to possess rejuvenating, anti-ageing and spermatogenic effects. [1] Seeds contain the highest levels of psychoactive constituents while leaves and roots in India are commonly used as antiseptic and anti-inflammatory agents. In Unani medicine, the roots are described as aphrodisiac and diuretic and are used for gonorrhoea. The leaves function as antiphlogistic emollients, local stimulants, rubefacients, and other dermatological. Leaf extracts are administered internally for boils and swellings, while external applications are used for eczema, ulcers, ringworm, and other skin conditions. The seeds function as hypotensive, spasmolytic, and general tonics. [2] Additional reports include Anti-convulsant and nootropic activities [3,4]. Topical ethanol extract of Argyeria nervosa leaves significantly accelerates wound healing in both normal and diabetic foot models. [5,6] Scientific Classification Key Taxonomical Details[32] Vernacular name Gujarati: Samundrasosh, Vardharo Hindi: Samandar-ka-pat, Samundarsokha, Ghav-patta Bengal: Bichtarak, Guguli Malayalam: Samudrapachcha, Samudrapala, Samudrastokam Marathi: Samandarshokh, Samudrasoka Sanskrit: Antakotarapushpi, Chhagalanghhri, Vryddhadaraka, Sam MORPHOLOGY Root Argyeria nervosa is often known as elephant creeper is a type of plant that is generally tells it age through its texture. To examine its roots and stems it is distinguished in the following way. The roots generally vary significantly depending on its their maturity. Younger roots: They usually measure only up to 2 to 4mm and are quite delicate. They have smooth and brownish skin. The younger roots when cut into a slice generally display a dark ring right in the middle . Mature roots: when the plant ages the roots are thicken reaching up to 25mm or more. The root skin becomes rough and bumpy due to a high concentration of small pores. The older roots include a colourless tertiary phloem in the internal structure which is paired with a pinkish, crescent-shaped “tertiary xylem”. The stems undergo a similar transformation as it climbs. Early stage: when the stem is young, it appears pure white and feels soft to touch. Maturity: when the stem reaches about 25mm in thickness, it loses its soft appearance. But it develops deep vertical ridges and is covered in elongated pores[26,30]. Leaf The lower surface of the leaf is fully covered with hair, which gives the leaf silver a soft woolly appearance. The upper surface of the leaf is generally green, glorious and also shows the marking of nerves by slight depressions. The mature leaf is Dorsiventral, Unicostate with a midnerve and several lateral nerves which are cordate at the base. The margin is entire but slightly wavy near the base. On the midrib 14-20 pairs of lateral nerves arises and on the neighbouring nerve the anterior branch unites with the posterior branch which are connected by an arched nervule[26,31]. Seeds The seeds of Argyeria nervosa are commonly known as Hawaiian Baby Wood Rose. They are easily identified by their distinct, roughly triangular shape which include two flat or slightly concave faces and one round face. Leafs measure roughly 0.5 to 0.75cm in length and up to 5mm in width. The seeds have a generally hard, stony texture which makes them difficult to break easily. It’s include a hilum generally a brown, circular mark nestled within a small depression at the seeds wider end. The surface of the leafs is generally smooth, and often dusted with persistent patches of white, papery pulp[26,29]. 2.Phytochemical constituents of Argyeria nervosa The following table summarizing the major phytochemicals classes found in Argyeria nervosa and their associated biological activities along with its wound healing capability. These plants secondary metabolites play crucial roles in environmental interaction and defence form the basis of many traditional and modern medicines. 3. Pharmacological Activities of Argyeria nervosa. 1. Argyeria nervosa (family Convolvulaceae), commonly known as Hawaiian baby wood rose, is a medicinal plant known for its rich content of ergoline alkaloids and diverse pharmacological properties[7]. 2. The plant contains lysergic acid amide (LSA), lysergic acid hydroxyethyl amide (LSH), ergometrine, chanoclavine, elymoclavine, agroclavine, flavonoids, tannins, phenolic compounds, saponins, and glycosides[8]. 3. Central Nervous System Activity LSA acts as a partial agonist at serotonin receptors, particularly 5HTA, 5HTA, and 5HTC receptors. This leads to modulation of thalamocortical signalling, altered sensory perception, mood changes, and cognition[9]. 4. Sedative and Anxiolytic Effects Partial agonism at 5HTA receptors and indirect modulation of GABAergic pathways result in reduced anxiety and sedative effects at lower doses[10]. Conclusion Argyeria nervosa stands with ayurvedic wisdom and modern pharmaceutical sciences. Traditionally, it is used as a memory enhancer, improves reproductive health, and supports vitality. In modern Pharmaceutical research, it yields results in the field of green nanotechnology, such as antibacterial and anticancer-treating nanoparticles. If it is not used efficiently and the extraction and use of Argyria nervosa is not done safely, it can be lethal due to its high content of alkaloids. Argyeria nervosa shows huge potential in treating and curing diseases, but it requires a transition from animal studies to human clinical studies and standardised dosing to unlock its full potential without any risk. References

Morphological diversity of Lasiodiplodia sp. isolates infecting woody and fruit crops in Côte d’Ivoire

Yaya Kone¹
Akissi Sandrine Yao¹
Adjoa Marie Josephine Kouadia¹
Massiata Dagnogo¹
Léon Etien Hamian²
Eric-Olivier Tienebo¹
Kouakou Théodore Kouadio¹
Kouabenan Abo¹

1. INTRODUCTION Fungal diseases pose a major threat to agricultural production in tropical regions, affecting both the quality and yield of woody and fruit crops [1]. In Côte d’Ivoire, crops such as cashew (Anacardium occidentale), mango (Mangifera indica), cacao (Theobroma cacao), and rubber (Hevea brasiliensis) are important sources of income for local producers. However, the productivity of these crops is frequently limited by fungal diseases, including those caused by Lasiodiplodia [6]. Lasiodiplodia spp. is a phytopathogenic fungus widely distributed in tropical and subtropical zones, responsible for fruit rot, twig dieback, and cankers on many woody trees [12]. This genus is distinguished by its high morphological and physiological diversity, influenced by the host species, growing environment, and agroecological conditions [8,11]. In Côte d’Ivoire, despite some studies on *Lasiodiplodia theobromae* in cocoa and mango plantations [3], knowledge of the morphological diversity, distribution, and pathogenic potential of isolates from different crops remains limited. Morpho-cultural and microscopic characterization of isolates is an essential tool for understanding the variability of fungal populations and adapting integrated disease management strategies [4,15]. Indeed, the diversity of traits such as mycelial growth, pycnid production, and conidia density and morphology can reflect the genetic variability of isolates and their ability to adapt to environmental conditions [14]. Thus, this study aims to evaluate the distribution and morphological and cultural diversity of Lasiodiplodia sp. isolates collected from different woody and fruit crops in Côte d’Ivoire, in order to provide useful information for disease management and the improvement of agricultural productivity. 2. MATERIALS AND METHODS 2.1. Prospecting and sample collection Surveys were conducted during the production season in the main agro-ecological and agricultural production areas of Côte d’Ivoire. They covered several crops of economic interest, including cashew, mango, cocoa, cola, rubber, Accra Cone, and banana (Figure 1). Plant material exhibiting characteristic symptoms, including dieback, cracking with exposure of internal tissues, gummy exudations, and rot were collected in the field. A total of 281 samples were collected from several locations distributed across different production regions, depending on the crops studied (Table 1). The collection covered 96 locations for cashew, 50 for cola (Cola nitida), 38 for cocoa (Theobroma cacao), 34 for mango (Mangifera indica), 29 for rubber (Hevea brasiliensis), 11 for Accra Cone (Polyalthia longifolia), and 11 for banana (Musa acuminata), distributed respectively across 7 to 15 regions depending on the crop. Cocoa pods naturally affected by black rot were collected from different trees within each surveyed plot and individually packaged in labeled plastic bags (date, plot code, and sample number). The banana samples, harvested at physiological maturity, from industrial plantations were obtained in Zambakro, Abengourou, Tiassalé, Dabou, Béoumi, Azaguié, and Agboville. A detailed breakdown of the samples by crop, location, and region is presented in Table 1. The collected samples were kept in plastic bags and transported to the phytopathology laboratory of the INP-HB in Yamoussoukro. 2.2. Isolation and purification of fungi The isolation and purification of fungi were carried out in the laboratory according to protocols adapted to the plant organs studied. For stem samples, the method described by [19] was applied with slight modifications. Symptomatic fragments of mango, rubber, cola, Accra Cone, and cashew stems (≈ 1 cm) were superficially disinfected in a 50% sodium hypochlorite solution for 1 min, then rinsed three times with sterile distilled water before being placed on PDA medium amended with citric acid. The harvested bananas were washed, rinsed with sterile distilled water, and kept at 28°C. Ripening was homogenized by immersion in an ethylene solution (2 ml·L⁻¹) according to [9]. After lesions appeared, the fruits were disinfected with 70% alcohol, and fragments taken from the growth front were inoculated onto PDA medium. For cocoa, subcortical tissue fragments were collected from necrotic lesions of pods affected by black rot, after disinfection with 96% alcohol and flaming, and then placed on PDA medium [5]. Petri dishes were incubated in the dark for 3 days at 28 ± 2 °C. Isolates were purified by subculturing at the mycelial growth front [5]. 2.3. Laboratory identification The final stage of the study consisted of identifying the pathogens associated with fruit dieback and rot. Isolates were characterized based on morphological criteria, including macroscopic observation of colonies (color, general appearance, and presence of pycnidia) and microscopic examination of conidia (morphology, color, and size). Species identification was performed by referring to the descriptions and taxonomic keys proposed by [16]. 2.4. Morphological characterization of the isolates In total, 161 isolates representative of the different sampling areas were selected for the evaluation of morpho-cultural parameters. The study was carried out on three culture media: PDA, MEA and agar, prepared according to the protocols described by [18] for PDA and agar media, and by [13] for the MEA medium [8]. For each isolate, a 4–5 mm diameter mycelial explant, taken from the growth front of a 5-day-old culture on PDA, was placed in the center of Petri dishes containing the different media. Each isolate was subcultured into five dishes (replicates) and incubated at 28 ± 2 °C under a 12 h/12 h light/dark cycle, as described by [16]. Morphological and cultural traits were assessed based on mycelial growth rate, as well as mycelium color and texture. Radial growth was measured daily by drawing two perpendicular axes on the back of each Petri dish and then calculating the average of the two diameters until the dish was fully filled [7]. The average growth of each isolate was determined from the five replicates. The diameter (in mm) of each isolate was measured in two perpendicular directions, and the average colony diameter was calculated using equation 1. where D is the average diameter of the isolate in a box, d1 and d2 are the measurements of the two perpendicular diameters. Growth was measured daily until full culture expansion using equation 2: where CM is the daily mycelial growth rate, Ddn is the average diameter growth on the day of measurement. Dd0 = initial diameter of the mycelium disc, which is 5 mm [8]. The appearance of colonies and … Read more

Analysis of Cashew Tree Yield Variability at the Tree and Orchard Scales

Gabésongon Kone¹
Abo Kouabenan¹
Mariam Bognan Coulibaly²

Introduction The cashew tree (Anacardium occidentale L.), a perennial species belonging to the Anacardiaceae family, is native to northeastern Brazil. Widely recognized for the significant economic value of its fruit—the cashew nut—the species was introduced to Africa and Asia by the Portuguese [1]. Although initially deployed to combat soil erosion, it has since evolved into a major cash crop across West Africa [2]. This transition is driven by its high market value, the increasing organization of the value chain, and its vital socioeconomic role in rural communities. Currently cultivated in nearly all tropical regions, cashew nuts hold a strategic position in Côte d’Ivoire, which emerged as the world’s leading producer of raw cashew nuts (RCN) in 2019 [3]. By 2023, Ivorian production reached an estimated 1,225,935 tons, accounting for approximately 40% of global output [4]. The cashew tree plays a fundamental role in the socioeconomic landscape of rural populations in Côte d’Ivoire. Much like cocoa, its cultivation enables the northern and northeastern regions to bolster financial resources and improve access to essential education and healthcare services. Cashew farming is predominantly characterized by smallholder family farms, with plots typically ranging from 0.5 to 3 hectares. These systems frequently employ intercropping with food crops, a strategy that not only optimizes land utilization but also reduces maintenance costs; specifically, the expanding canopy of the cashew trees naturally suppresses weed growth. Revenue generated from the marketing of cashew nuts is further utilized to fund social ceremonies (such as weddings, funerals, and rituals), acquire consumer goods (including motorcycles and appliances), and invest in housing construction [5]. Overall, the cashew sector supports approximately 2.5 million Ivorians and contributes 7% to the national Gross Domestic Product (GDP) [5]. Despite its socioeconomic importance, the average yield per hectare remains relatively low, estimated at 620 kg/ha, which is significantly below the optimal potential of 1,200 kg/ha for raw cashew nuts (RCN). This yield gap is attributed to several factors, including insufficient agricultural investment, limited knowledge regarding input application, and a lack of proficiency in essential cultural practices, such as pruning. Previous studies have highlighted these challenges, emphasizing that specific agricultural behaviors and technical constraints directly contribute to the low productivity of orchards across the region [7, 8, 9]. Notwithstanding the alarming data on low yields, comprehensive knowledge of tree cropping systems in West Africa remains limited and fragmented. Whether at the micro-scale (individual tree) or the macro-scale (plot or production basin), available data are frequently imprecise, incomplete, or absent In Côte d’Ivoire, while cashew nuts occupy a strategic position in rural economies—serving as an essential income source for producers in the north and northeast—the crop’s potential remains largely under-exploited. This underperformance is compounded by high yield variability, which remains poorly documented at both the tree and orchard levels. A deeper understanding of this variability is therefore critical to identifying primary limiting factors and developing tailored agronomic interventions. In this context, the present study aims to analyze cashew nut yield variability at two distinct levels: the individual tree and the orchard. Specifically, the objectives are to: Research Hypotheses H1: Tree productivity varies significantly based on age and planting density. H2: Tree morphology (height, trunk diameter, and canopy diameter) is significantly influenced by both planting density and tree age. METHODS Materials And Methods This section details the resources and procedures implemented to conduct the study. It is organized into two primary components: Materials, which include a description of the study site, plant material, and measurement tools; and Methods, which provide a detailed account of the data collection protocols and statistical analyses. Study Site The study was conducted across 24 orchards in the Poro region (8°26’–10°27′ N, 8°26’–10°27′ W). Covering an area of 13,400 km², the Poro region is organized into four departments: Dikodougou, Korhogo (the regional capital), M’Bengué, and Sinématiali. The regional climate is Sudanese, characterized by a distinct dry season from November to April and a rainy season from May to October [10]. Annual rainfall typically ranges from 1,000 to 1,400 mm, with average monthly temperatures between 26.93°C and 27.02°C, peaking at approximately 36°C in March [11]. The vegetation is diverse, featuring Sudanese savanna in the northern reaches and sub-Sudanese savanna in the south. The topography is varied, with elevations such as Mount Korhogo exceeding 500 m. Soils are predominantly ferralitic, ferruginous, and hydromorphic, characterized by high permeability and porosity. The local economy is primarily driven by livestock and agriculture, including major cash crops (cotton and cashew) as well as food crops (yam, rice, and sorghum). Materials This subsection details the equipment used, ranging from field data collection (morphological and yield parameters) to laboratory-based statistical processing. Plant Material The plant material consisted of cashew trees (Anacardium occidentale L.) located within 24 orchards delineated into study plots in the Poro region. Technical Equipment Cashew tree dimensions were measured using a decameter, while a graduated wooden pole was used for height measurements. Plot boundaries (2,500 m²) were established using ropes, and the seven selected sample trees per plot were marked with spray paint. For nut collection and measurement, harvest bags and a precision scale were used. Data (nut mass and quantity per tree and per plot) were initially recorded in field notebooks and subsequently digitized using Open Data Kit (ODK). Methods Orchard Selection To analyze cashew yield variability at both the tree and plot scales, four age categories were selected: 5, 10, 20, and 30 years. These categories were chosen to better understand yield fluctuations and tree physiological behavior over time. Data Collection Design A preliminary survey was conducted to assess orchard age and density. Consequently, the selection focused on these two factors. For each of the four age categories (5, 10, 20, and 30 years), three planting density classes were defined: Plot Establishment: Based on these criteria, 18 orchards were selected (2 orchards per density class × 3 density classes × 4 age categories — Note: Please verify if the total is 18 or 24 based on your math). Within each orchard, a 50 m × 50 m (2,500 m²) plot was established, and seven specific trees were monitored within each … Read more

Assessment of the Growth of Agronomic Parameters of Pumpkin (Curcubita pepo L.) to Rates of Poultry Manure

Onwubiko, G. N.
Okereke, C.S.

Introduction Vegetables are gradually acknowledged as crucial for food and nutrition security [22]. However, organic vegetables are increasingly preferable to many consumers as they are shifting their food choice priorities towards food they perceive to be healthier for themselves and the environment. Cucurbita pepo is a squash or pumpkin belonging to the Cucurbitaceae family and genus cucurbita that resembles a guard, with over 130 genera and up to 800 species [21]. In the agricultural industry, pumpkins are cultivated as vegetables on a large scale, contributing to the agricultural economy. The production and sale of pumpkins, whether for fresh consumption or processing into various products, generate revenue for farmers and the agricultural sector. They are processed into a wide range of food products such as pumpkin soup, pumpkin-based snacks, pumpkin pie filling, etcetera. These processed products contribute to the food industry’s revenue and provide employment opportunities in processing and manufacturing plants. The seasonal demand for pumpkins drives retail sales of fresh pumpkins, decorative pumpkins, and pumpkin-related products, contributing to the economy, especially in regions with a vibrant pumpkin culture. In some cultures, pumpkins are an important part of traditional dishes and celebrations, such as pumpkin pie in the USA, or pumpkin lanterns on Halloween. Pumpkin is a natural treasure full of health benefits, from its seeds to its flesh and even leaves; almost every part of pumpkin can be used for healing, nourishment, or even beauty. Pumpkin is highly nutritious and enriched in vitamins A, C, and E, dietary fiber, and potassium [11]. Pumpkin is rich in potassium, which helps to balance the effects of so much sodium in the body. These nutrients support overall health and well-being, making pumpkins a valuable addition to a nutritious diet. The fiber in pumpkin aids absorption of sugar in the bloodstream thus regulating blood sugar levels, supports digestion, and relieves constipation ([6]. Their vibrant orange colour is indicative of their high beta-carotene content, which helps to improve eyesight and reduce the risk of eye diseases like night blindness and age-related macular degeneration. These carotenoids possess properties associated with a reduced risk of certain chronic diseases, such as cardiovascular diseases and certain types of cancer, and support immune function [17,23]. Also, cucurbitacins, a group of compounds found in Cucurbita pepo, reportedly possess anti-inflammatory and potentially alleviate symptoms associated with inflammatory conditions such as arthritis and certain autoimmune disorders [7]. Cucurbita fruit is low in calories and provides many advantages for human health, including blood cleansing, constipation relief, improved digestion, and energy production [9]. Cucurbita seed kernels have been employed as functional materials and nutrient supplements in baking, cooking, and ground meat compositions [25,13]. It has been observed that the seed extract possesses antioxidant, antitumor, antibacterial, anticancer, and antimutagenic properties [7]. Strong hypo-triglyceridemic and serum cholesterol-lowering effects have also been observed [16]. The nutrients in pumpkin are essential for various bodily functions important for good health such as hydration and boosting immune support [26]. Its high-water content and antioxidants make it great for feeling full and boosting your body’s defense. Pumpkin seeds, also called pepitas, are used to reduce prostate enlargement. The high quantity of zinc in the seed can be used for the management of prostate problems. Pumpkin seed oil is mostly composed of linoleic acid, oleic acid, palmitic acid, tacophenol, ß-sitosterol, and delta-7-sterols. Pumpkin pulp is rich in nutrients and health-promoting properties such as proteins, carotene, mineral salts, vitamins, and polysaccharide-like proteins [8]. The significance of this crop in human life makes it crucial to research on and to boost its production to keep up with the rising demand for its products. Soil fertility is a significant issue that hinders the productivity of crops [19]. The optimum temperature range for pumpkin growth and development is paramount to determining yield and quality. Pumpkin plants prefers well-drained, loamy soils with a good balance of sand, silt, and clay and the optimum soil pH range is between 6.0 and 7.5 (slightly acidic to neutral soils). Additionally, environmental factors such as soil moisture, light intensity, and relative humidity, can also influence the plant’s growth and development [14]. Poultry manure is a well-established and desirable organic fertilizer that bolsters soil fertility by adding both essential nutrients and soil organic matter. Each of these components contribute positively to moisture and nutrient retention in the soil [15]. Of all animal-derived manures, chicken manure is considered best organic soil fertilizer forfor crops due to its richness in most of the major nutrients required for plant growth. Some of these qualities include microbiological activities, soil tilth, and chemical properties [28,29]. The application of poultry manure in record yielded over 53% increases in N level in the soil, from 0.09% to 0.14 % and exchangeable cations increased with manure application [10]. When added in the appropriate amount, poultry manure enhances soil quality and provides nutrients for crop growth because it is high in organic matter and other nutrients required for plant growth [27]. According to [12], adding poultry manure to the soil enhanced its water-soluble qualities and carbon content while decreasing its bulk density. The ideal rates of poultry manure application can vary based on soil nutrient levels, manure source/composition, and specific cultivar needs. Having a soil test done is recommended to determine precise fertilizer requirements when using manures and over application of the poultry manure can lead to nutrient imbalances or salt issues. Poultry manure is considered a high-nitrogen fertilizer, and too much application can lead to excessive vegetative growth at the expense of fruiting. Poultry manure is abundant in nitrogen (3-4% N) and moderate in phosphorus (2-5% P2O5). It is low in potassium, so supplementing with K may be needed, especially for fruiting. The nutrient ratio is around 3-2-1 for N-P-K in poultry manure. It was previously reported that applying poultry manure at rates up to 20 tons/ha significantly improved pumpkin plants’ growth and yield parameters, including vine length, number of leaves, number of branches, leaf length, and stem girth [2]. In a later report, poultry manure application promoted early flowering and … Read more

Potassium Use Efficiency of Modern Rice Variety Influenced by Integrated Nutrient Management Options in Dry Season

Chowdhury Md. Humayun Kabir¹
Alok Kumar Pau²
Biswajit Karmakar³
Israt Alam⁴
Khalid Syfullah⁵

INTRODUCTION Rice (Oryza sativa L.) is the primary staple food for more than half of the global population and plays a crucial role in global food security and rural livelihoods [1, 2]. According to Food and Agriculture Organization, rice is cultivated on over 160 million hectares worldwide, producing more than 750 million tons annually [3, 4]. In Bangladesh, rice dominates the agricultural sector, occupying about 75% of total cropped area and contributing substantially to national food supply and farm income [5, 6]. Despite remarkable progress in varietal development and crop management, average rice yield in Bangladesh remains lower than that of major rice-producing countries due to nutrient imbalances, soil fertility decline, and inefficient fertilizer use. Potassium (K) is one of the three primary macronutrients required in large quantities for rice growth and productivity [7, 8, 9, and 10]. It plays key physiological roles in enzyme activation, photosynthesis, osmotic regulation, assimilate transport, and stress tolerance. Adequate potassium supply enhances resistance to lodging, pests, and diseases, improves grain filling, and increases yield stability [9]. Rice crops typically remove 100–300 kg K ha⁻¹ per season, yet K fertilization in many rice-growing areas is often inadequate relative to crop removal, leading to soil K depletion and declining productivity [11, 12]. Continuous intensive cropping, use of high-yielding varieties, and limited recycling of crop residues further aggravate potassium deficiency in South Asian soils. Improving potassium use efficiency (KUE) has therefore become essential for sustainable rice production[13,14,15]. Integrated nutrient management (INM), combining inorganic fertilizers with organic amendments such as cowdung or compost, has been widely recommended to enhance nutrient availability, improve soil physical properties, and increase fertilizer efficiency. Organic amendments can increase cation exchange capacity, reduce nutrient losses, stimulate microbial activity, and enhance K availability from non-exchangeable soil pools. However, information on KUE of modern high-yielding rice varieties under different integrated nutrient management options during the dry (Boro) season remains limited[3]. Modern rice varieties developed by Bangladesh Rice Research Institute have higher yield potential but also greater nutrient demand. Efficient nutrient management strategies are therefore required to achieve high productivity without degrading soil health. Understanding potassium uptake dynamics and use efficiency under different fertilization strategies will help optimize fertilizer recommendations and improve sustainability of rice-based systems [16,17,18]. Therefore, the present study was conducted to evaluate the growth performance, yield, potassium uptake, and potassium use efficiency of a modern rice variety under different fertilizer management options, including recommended practice, soil-test-based fertilization, and integrated nutrient management during the Boro season. The findings are expected to provide guidance for improving potassium management and sustaining rice productivity in intensive rice-growing regions. Materials and Methods Study Site The experiment was conducted during the Boro seasons of 2022 and 2023 at the research field of Sher-e-Bangla Agricultural University, located in Dhaka. The site belongs to the Agro-Ecological Zone (AEZ-28, Madhupur Tract) and is characterised by subtropical monsoon climate with mild winter and hot, humid summer. The experiment was carried out on typical rice-growing silty clay loam soil at the Soil Science Farm of Sher-e-Bangla Agricultural University. Surface soil samples (0–20 cm depth) were collected before land preparation and analysed for physicochemical propertiesThe soil contained 18.60% sand, 45.40% silt, and 36.00% clay, with a pH of 6.8, organic matter 1.38%, total N 0.06%, available P 19.85 mg kg⁻¹, exchangeable K 0.12 meq 100 g⁻¹, and available S 14.40 mg kg⁻¹. Experimental Design and Treatments The experiment was laid out in a Randomized Complete Block Design (RCBD) with three replications. Plot size was 3 m × 4 m, and plots were separated by 0.5 m bunds. Seven potassium management treatments were evaluated using the rice variety BRRI dhan100: Fertiliser sources included urea for N, triple superphosphate for P, muriate of potash for K, gypsum for S, and zinc sulfate for Zn. Cowdung was well-decomposed before application. Crop Establishment and Management Seeds of BRRI dhan100 were sown in well-prepared wet nursery beds on 11 December 2021 and 7 December 2022. Thirty-five-day-old seedlings were transplanted on 26 January 2022 and 13 January 2023 at 25 cm × 15 cm spacing with 2–3 seedlings per hill. Cowdung was incorporated 20 days before transplanting. The full doses of P, K, S, and Zn were applied during final land preparation, while nitrogen from urea was applied in three equal splits at 15, 30, and 45 days after transplanting. Standard agronomic practices including irrigation, weeding, and plant protection were applied uniformly across treatments. The crop was harvested at physiological maturity when approximately 85–90% of grains turned golden yellow. A central harvest area of 6 m² per plot was used to determine grain and straw yields. Grain yield was adjusted to 14% moisture content and straw yield to 3% moisture. Plant Sampling and Nutrient Analysis Grain and straw samples were oven-dried, ground, and analysed for potassium content. Digested samples were diluted to a known volume, and potassium concentration was determined using a flame photometer. Nutrient uptake was calculated by multiplying nutrient concentration with corresponding biomass yield. Potassium Use Efficiency Indices Potassium use efficiency indices were calculated using standard equations: Partial Factor Productivity of K (PFPK) PFPK (kg kg-1) = GY+K/FK [18] Agronomic Efficiency of K (AEK) AEK (kg kg-1) = (GY+K – GY0K)/FK [18] Recovery Efficiency of K (REK) REK = (UK+K – UK0K)/FK [18] Physiological Efficiency of K (PEK) PEK (kg kg-1) = (GY+K – GY0K)/(UN+K – UN0K) [18,19] Internal Efficiency of K (IEK) IEK = GY/UK [19] Potassium Harvest Index (KHI) NHI = GK/TK [18,19,20] where GY+K and GY0K are grain yields with and without potassium, FKis applied potassium, U+Kand U0K are potassium uptake with and without fertiliser, GK is grain potassium uptake, and TK is total plant potassium uptake. Statistical Analysis Data were analysed using analysis of variance (ANOVA) appropriate for RCBD. Treatment means were separated using Least Significant Difference (LSD) at 5% probability level [21]. Statistical analyses were performed using Statistix 10. Experimental Validity The experiment was repeated across two consecutive years to ensure reproducibility and minimise seasonal variability. Uniform crop management … Read more