Cucurbita pepo is a squash belonging to the Cucurbitaceae family and genus Cucurbita that resembles a gourd. It is an annual crop native to Mexico but cultivated worldwide for its fruit, leaves, and seeds used in cooking and in medicine. The use of poultry manure as an organic fertilizer significantly impacts Cucurbita pepo growth and yield. Poultry manure (pm) is enriched in nitrogen, phosphorus, and potassium, as well as various micronutrients which promote plant growth and development. An experiment to determine the growth response of pumpkin (Cucurbita pepo L.) to different rates of poultry manure was conducted at the teaching and research farm of the Faculty of Agriculture, University of Nigeria, Nsukka. The treatments used were poultry manure applied at 0t/ha, 5t/ha, 10t/ha, and 15t/ha. The experiment was laid out in a Randomized Complete Block Design (RCBD), with four (4) treatments and three (3) replications per condition assessed. Data collected included vine length, number of leaves, leaf length, number of branches, and stem girth at certain growth stages. Data analysis for statistical significance was obtained via analysis of variance (ANOVA) test using GenStat, a statistical software package. Separation of treatment means was done using Fisher’s Least Significant Differences (F-LSD) at 5% probability level. Significant effect at (P≤0.05) for vine length, leaf length, number of leaves, and number of branches per plant was observed, but generally, poultry manure at 15t/ha showed the highest value on most of the parameters studied. The treatment with the application of zero poultry manure gave the highest percentage of moisture loss. For sustainable pumpkin production, the application of poultry manure at 15t/ha is suggested for use in pumpkin production, as pumpkin treated with poultry manure at that level produces significantly higher organic yield than any other treatment levels.