Volume 4 Issue 1 2025 – Journal of Plant Biota

Triticale Varieties: The Effect of Fertilizer Rates on Changes in Leaf Pigment Content

Maftuna Raimqulova¹
Akmal Sanakulov¹
Mahliyo Usmonova²

Introduction Nowadays, the rapid growth of the world population is leading to an increased global demand for food. As a result, the need for plant-based products is also rising. In this context, special attention is being paid to the evaluation and application of the physiological and biochemical characteristics of new plant species with high productivity and nutritional potential, especially in arid and water-deficient regions. Research conducted in this direction is considered a solution to some of the most important and urgent problems. Therefore, studies aimed at producing high-quality, low-cost, and environmentally friendly agricultural products are among the most pressing issues today [1, 2]. Triticale (× Triticosecale) is a man-made cereal crop developed by crossing wheat and rye [2]. The name “Triticale” is derived from the combination of the scientific names of wheat (Triticum) and rye (Secale) [6]. The first hybridization was carried out in the late 1870s in Scotland, and the first fertile wheat–rye amphidiploids were obtained in 1888 by the German scientist Rimpau. A widespread triticale improvement program began in 1964, and today it is considered the main supplier of improved germplasm for national and international programs worldwide [11, 3, 8]. Triticale is a crop that is resistant to adverse environmental conditions, diseases, and high temperatures, and it adapts well to unfavorable soils and climates [5, 13]. Currently, triticale occupies a strong position in agricultural production across various countries [4]. It is mainly cultivated for grain feed and green fodder. However, in recent years, triticale grain has increasingly been used in the food and alcoholic beverage industries [7]. Photosynthesis occurs primarily in green leaves, and to a lesser extent in young shoots and unripe fruits, due to the presence of chloroplasts. Pigments found in plant tissues play a vital role in this process. In particular, chlorophylls are directly involved in all primary reactions of photosynthesis [9, 15]. Literature Review In Uzbekistan, research on cultivating autumn triticale as a forage crop on irrigated lands, including studies on optimal sowing times, seeding rates, and fertilizer norms, has been conducted by I.V. Massino (1989). On rainfed lands, studies by M. Khayitboev (2019) and K.T. Isokov (2020) have focused on developing early-maturing breeding materials to ensure stable yields. J. Doschanov (2021) and colleagues have assessed the salt tolerance of triticale cultivars, highlighting growing scientific interest in this crop. Several foreign and CIS scientists have also made significant contributions to triticale research. These include P. Gupta (1982), M. Mergoom (2004), A. Blum (2014), R. Agil (2014), E. Arseniuk (2015), and V.Yu. Kovtunenko (2019). In the CIS, contributions have been made by A.I. Grabovets (2000), N.V. Rogozhkina (2006), N.N. Zezin (2010), S.A. Gorchin (2012), T.A. Goryanina (2017), N.P. Shishlova (2018), and B.B. Boronchikhin (2019). Research Object and Applied Methods The triticale cultivars Odyssey and Sardor were selected as the experimental objects. The study was conducted under field conditions in the Samarkand region during the 2024–2025 growing seasons. All analyses, phenological observations, and calculations were carried out in accordance with established methodological guidelines. The content of pigments in the leaves was determined using the method developed by V.F. Gavrilenko, M.E. Ladigina, and L.M. Khandobin, utilizing an SF-26 spectrophotometer. Results and Discussion Chlorophyll a and b are considered the primary pigments involved in the photosynthesis process. Additionally, carotenoid pigments—yellow, orange, and red—are present and play key roles in various physiological functions. Carotenoids protect plants from harmful environmental factors and help transfer short-wavelength light energy to chlorophyll molecules [12, 14, 16]. It was found that the content of plastid pigments and carotenoids in the leaves of triticale cultivars Odyssey and Sardor varied depending on the nitrogen fertilizer rates. The experimental data are presented in Tables 1 and 2. The amount of plastid pigments in fresh triticale leaves changed across the different growth stages. Total chlorophyll content increased from the tillering stage to the flowering stage, then decreased during the grain ripening stage. It was also revealed that the amount of chlorophyll a was higher than that of chlorophyll b in both triticale cultivars studied. Content of plastid pigments (mg/g) in the leaves of the Sardor cultivar at different growth stages The amount of carotenoid pigments was initially lower than that of chlorophyll a and b, but it gradually increased throughout the vegetation period. In the Sardor cultivar of triticale, during the tillering stage, total chlorophyll content varied across treatments from 4.31 mg/g to 5.12 mg/g. Chlorophyll a ranged from 2.52 mg/g to 2.99 mg/g, while chlorophyll b ranged from 1.79 mg/g to 2.13 mg/g. Carotenoid content varied from 1.51 mg/g to 1.84 mg/g depending on the fertilizer treatment. The ratio of total chlorophyll (a + b) to carotenoids ranged between 2.85 and 2.93. During the stem elongation stage, the total chlorophyll content in the Sardor cultivar increased, ranging from 4.69 mg/g to 5.91 mg/g. Chlorophyll a content ranged from 2.81 mg/g to 3.22 mg/g, and chlorophyll b from 1.88 mg/g to 2.69 mg/g. Carotenoid content was lowest in the control (1.57 mg/g) and highest in the N300 P210K150 treatment (1.92 mg/g). Intermediate values were observed in the other variants. The chlorophyll a + b to carotenoid ratio ranged from 2.98 to 3.07. At the heading stage, further increases in pigment content were recorded. Chlorophyll a was lowest in the control (3.04 mg/g) and highest in N300 P210K150 (3.56 mg/g). Chlorophyll b ranged from 2.34 mg/g (control) to 2.75 mg/g (N300 P210K150). Carotenoid levels remained lower than those of chlorophylls, ranging from 1.61 mg/g to 1.99 mg/g. Total chlorophyll content increased from 5.38 mg/g (control) to 6.31 mg/g (N300 P210K150). The ratio of total chlorophyll (a + b) to carotenoids ranged from 3.11 to 3.34. During the flowering stage, total chlorophyll content reached its peak compared to the other growth phases. In all treatments, pigment content increased. Total chlorophyll levels were 5.53 mg/g in the control (0.15 mg/g higher than at the heading stage), 5.87 mg/g in N150P105K75 (+0.26 mg/g), 6.36 mg/g in N200 P140K100 (+0.30 mg/g), 6.13 mg/g in N250 P175K125 (+0.49 mg/g), and 6.59 mg/g in … Read more

Performance evaluation of aggregatum onion genotypes (Allium cepa var.aggregatum) for yield and quality under Coimbatore conditions in Tamil Nadu

Usha Nandhini Devi Harinarayanan¹
Pugalendhi Lakshmanan²

1.Introduction Aggregatum group includes shallots and potato onions and are referred to as multiplier onions. The bulbs are smaller than those of common onions, and a single plant forms an aggregate cluster of several bulbs from a central bulb. They are propagated commercially from daughter bulbs, and varieties which set seeds under TamilNadu conditions are propagated through seeds. Shallots are the most important subgroup within this group and comprise the only cultivars cultivated commercially. They form aggregate clusters of small, narrowly ovoid to pear-shaped bulbs. Potato onions differ from shallots in forming larger bulbs with fewer bulbs per cluster, and having a flattened (onion-like) shape. Intermediate forms exist [5]. Diversity in plant genetic resources offers plant breeders the opportunity to develop novel and better cultivars with desirable characteristics, which include both farmer-preferred traits like yield potential and larger bulbs and breeder-preferred traits which include pest and disease resistance and photosensitivity [6]. The conservation of germplasm is necessary for maintaining genetic diversity, studying local genetic material and under-utilized species, and to choose species suitable for developing newer varieties [3]. No country in the world is self-sufficient in germplasm to fulfill its food requirements. Many countries predominantly depend on nonindigenous crops and imported germplasm for food and agricultural development [4]. Aggregatum onion (Allium cepa var. aggregatum) is mainly grown in Tamil Nadu, Andhra Pradesh and Karnataka. In TamilNadu it is cultivated in an area of 30,000 ha. It is well known for its use in sambar preparation, an important South Indian dish. It has demand in the international market due to its unique taste and flavor. Inorder to identify genotypes which perform better in terms of quality and yield, this study has been undertaken to evaluate the performance of genotypes available. 2.Materials and methods The present study was conducted at the College Orchard of the Department of Vegetable Science, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore during 2021-2022. A total of 65 genotypes maintained at the Department of Vegetable Science were evaluated for yield, quality parameters, and disease index. (Table.1) The experiment was laid in randomized block design with two replications. The package of practices as recommemded by the crop production guide of TamilNadu was followed. Observations were recorded on Average bulb weight (g), Total Soluble Solids (%), Total Yield (t/ha),thrips damage(%), leaf spot (PDI) and Stemphyllium blight (PDI). Ten plants were randomly selected from each plot for recording data and the average was calculated. After curing, the total bulb fresh weight was measured for each plot. The total soluble solids (TSS) content of bulbs were recorded by hand refractometer. The disease (leaf spot and Stemphyllium blight) severity of onion was scored by following 0-5 scale, as described by [8]. The details of scales are as follows: 0-no disease symptoms, 1- a few spots towards tip covering 10% leaf area, 2- several dark purplish brown patch covering up to 20% leaf area, 3- several patches with paler outer zone covering up to 40% leaf area, 4- leaf streaks covering up to 75% leaf area or breaking of the leaves from center and 5- complete drying of the leaves or breaking of the leaves from the centre. Likewise, thrips infestation was also rated by following 0-5 scale. Observations were made at the first appearance of disease symptoms/thrips on plants, till the harvest at weekly intervals. Color of bulbs were identified by visual assessment method. 3.Results and Discussion The genotypes used in the study exhibited a wide range of differences in the parameters measured. The observations recorded are given in Table 2. 3.1. Bulb weight There were significant differences in bulb weight among the different genotypes (Table 2). The heaviest bulb weight (66.2g) was observed in the genotype Agg-Sel-1 followed by followed by Agg-Sel-3 with 64.4g. The lightest individual bulb (33.2 g) was observed in Agg-1519. The difference in bulb weight could be attributed to the genetic potential of the different genotypes. The results of the present study confirms with the findings of [9]. 3.2.Total soluble solid content The total soluble solids (TSS) content differed significantly among. The highest percent of Total Soluble Solids (15.3) was recorded in Agg1552 followed by Agg 1535 with 15.2% and Agg-Sel-4 with 15.0%. The lowest TSS content (7.0%) was observed in 1548-Agg.The variation in TSS content among the genotypes might be attributed to differences in the genetic potential of the genotypes used in the study. This is following the results of [1]. 3.3.Yield The genotypes expressed significant variation in yield per hectare. The genotypes, Agg 1522 and Agg 1692 recorded a total yield of 17.4t/ha which was higher than all other genotypes. The lowest yield per hectare (6.80t/ha) was observed in Agg-Sel-43 followed by Agg 1523 with 10.5t/ha. The superior performance of the genotypes could be due to their higher individual bulb weight. Similar results have been obtained by [2,7,10]. 3.4.Skin and flesh colour of the bulb The result of color measurement revealed that there was not much variation in the skin colour of the bulbs among the different genotypes evaluated. 3.5.Disease incidence and thrips infestation The genotypes evaluated also showed significant differences for disease scoring. The lowest percent of thrips (12.5) was observed in Agg 1528, the lowest leaf spot incidence was observed in Agg1532 with 11.5 and the lowest incidence of Stemphyllium blight was recorded in Agg-Sel-2 with 3.7.The apparent cause of the variation in disease severity and pest infestation expressed by the different genotypes might have been due to their genetic potential. 4.Conclusion Based on the present study it can be concluded that the genotypes, Agg-Sel-1, Agg-Sel-3 Agg 1522, and Agg 1692 recorded higher yield compared to other genotypes and the genotypes, Agg 1528, Agg1532 and Agg-Sel-2 showed lesser disease incidence and pest infestation. Hence these genotypes showing better performance can be utilized for further breeding programmes. Declaration of competing interest The authors declare that they have no conflict of interest. Declaration of generative AI and AI-assisted technologies in the writing process. The authors declare that they have not used AI … Read more

The Effect of Inorganic Fertilizer and Liquid Fertilizer Applications on the Growth and Yield of Maize (Zea Mays L.) in Makurdi, Nigeria

Iorlamen, T.
Ugoo, T. R
Madina, P.

1.0 INTRODUCTION Soil nutrient depletion is a global environmental issue that poses significant threats to food security and soil health [21]. It refers to the decline in soil organic matter and essential nutrient levels [28, 21], which can result in decreased crop productivity and the degradation of arable land. To guarantee sustained agricultural output and protect the environment, it is crucial to maintain and enhance soil quality [17]. Maize production in Africa’s sub-Saharan region has been significantly constrained by a variety of factors, with climate change and declining soil fertility being the most notable challenges. Among these, poor soil fertility stands out as the most persistent and pressing issue that has plagued the region for decades. This persistent degradation of soil health has played a central role in exacerbating food insecurity, as reflected in the steadily decreasing food production per capita, especially among smallholder farmers [24, 4, 19, 21, 7]. The causes of low soil fertility in SSA are multifaceted, involving both human-induced and natural processes. Human activities such as overgrazing, widespread deforestation, and uncontrolled removal of vegetation have contributed to soil degradation. Additionally, natural processes such as wind and water erosion play a significant role, stripping away vital nutrients like nitrogen and phosphorus from the soil. Leaching, particularly of nitrogen and potassium, due to heavy rainfall or poor soil structure, also contributes to nutrient losses in tropical regions, including SSA [33]. Furthermore, unsustainable agricultural practices especially the frequent use of continuous cropping systems without proper soil management have led to progressive nutrient depletion. The situation is worsened by the limited or incorrect use of fertilizers, which fails to replenish the lost nutrients effectively. These practices collectively diminish soil productivity and threaten the long-term sustainability of agriculture in the region [6]. Fertilizers, whether organic or inorganic, are substances applied to soil to provide essential nutrients that support plant growth and enhance soil health [5]. Their use has become a vital agricultural practice, significantly boosting crop productivity and encouraging the adoption of other sustainable farming methods. Consequently, fertilizer application is now a central component of many agricultural development programs across the globe [7]. According to [22], improving soil fertility is fundamental for increasing agricultural output. Enhanced soil fertility not only contributes to greater food security but also boosts farmers’ incomes. To address nutrient loss in soils, it is essential to apply fertilizers, either organic or inorganic, in proper quantities and using the correct techniques [2, 21, 7]. Researchers have extensively explored the use of various soil amendments as a strategy to combat declining fertility [34, 16, 25]. Inorganic fertilizers are known to improve crop yields, adjust soil pH, raise total nutrient levels, and increase nutrient availability. On the other hand, organic fertilizers such as animal manure enhance soil structure and maintain long-term fertility, especially under continuous cropping systems like maize cultivation [26]. Maize (Zea mays) is a vital staple crop in sub-Saharan Africa (SSA) and is considered the most significant cereal in the region, with Nigeria leading as the top maize producer on the continent [14]. Over time, maize has gained prominence, gradually replacing traditional grains like millet and sorghum in many areas [19]. In 2018, Nigeria produced approximately 10.2 million tons of maize from 4.8 million hectares of farmland, positioning it as Africa’s largest maize producer [11]. Advances in agricultural research have contributed to the development of improved technologies, including high-yielding maize varieties with resistance to drought, diseases, low soil nitrogen, and parasitic weeds like Striga [20, 19, 7]. However, despite the availability of these improved varieties, maize yields in Nigeria’s savanna regions remain low. This is largely due to declining soil fertility, a result of increasing pressure on land resources driven by population growth and limited fertilizer application [19]. Soils in these regions are often depleted of essential macronutrients such as nitrogen (N), phosphorus (P), and potassium (K), as well as important micronutrients like copper and zinc. Without adequate fertilization, the soil cannot support sustainable maize production, and yields may drop below 1 ton per hectare [10, 19, 7]. Given the strategic importance of maize, it is crucial to sustain its production at sufficient levels to support food security and self-sufficiency at both household and national scales [30]. To achieve this, efforts must be directed toward enhancing maize productivity by improving the soil’s physical and chemical properties. This improvement can be accomplished through the adoption of effective farming practices, including the application of suitable fertilizers and the implementation of sound agronomic techniques. In light of this, the present study assessed the effectiveness of combining inorganic fertilizers with liquid fertilizer foliar sprays in influencing the growth and yield performance of maize cultivated in Makurdi, located within Nigeria’s Southern Guinea Savannah ecological zone. 2.0 ‘MATERIALS AND METHODS’ 2.1 Experimental Site’ The study was carried out at Joseph Sarwuan Tarka University Makurdi’s (JOSTUM) Teaching and Research Farm in Nigeria during the late seasons (August to November) of 2023 and 2024. The research location, located at latitude 7.41°N and longitude 8.28°E, is 98 meters above sea level in the Southern Guinea Savannah ‘agro-ecological’ zone of equatorial Nigeria’. 2.2 Experimental Treatments and Design The experiment was conducted on a manually cleared field. A drought-tolerant maize variety, EVGT 2009, sourced from the Institute of Agricultural Research and Training in Zaria, was used. Fertilizers applied included NPK 20:10:10, Urea, and AgriBoom liquid fertilizer obtained from the registered agro-dealer in Makurdi. The study employed a randomized complete block design with five treatments: NPK (200kg/ha), NPK (200kg/ha) and AgriLife AgriBoom(1ltr/ha), NPK (200kg/ha) and AgriLife AgriBoom(2ltr/ha), AgriLife AgriBoom(3ltr/ha), and NPK (200kg/ha) and Urea (100kg/ha) as control and each treatment was replicated three times. Maize was planted at three seeds per hill, spaced 75 x 50 cm, and thinned to two plants per hill two weeks after sowing (WAS), targeting 53,333 plants per hectare. NPK was applied two weeks after planting, AgriLife AgriBoomliquid fertilizer foliar application at 4 weeks, 6 weeks, and 8 weeks after planting weeks after planting. Manual weeding was done at 3 and 6 WAS. All … Read more

Floral Biology Of Strobilanthes Callosus (Nees) Bremek.: An Underutilized Plant Species Of The Western Ghats

Shinde, H. P.¹
Salve, S. S¹
Chavan, S. N.¹
Korde, S. D.¹
Waghmode, A. V. ²
Samudra, S. M.³

INTRODUCTION Plantbiologists have long been fascinated by the diversity in floral morphology among plant species, prompting the creation of a number of explanatory ideas. [46]. The genus name originates from the Greek ‘Strobilos’ (cone) and ‘anthos’ (flower), reflecting the floral architecture. Strobilanthes is the second largest genus in Acanthaceae, comprising over 350-454 accepted species worldwide [26], with around 150 species recorded in India [43], primarily in the Western Ghats and Nilgiri hills [4]. One of its most fascinating members is Strobilanthes callosus Nees. (syn. S. callosa), a shrub endemic to the open hill slopes and valleys of Maharashtra and parts of peninsular India [24]. Documented from districts including Ahmednagar, Satara, Ratnagiri, Pune, and Nasik [42], the species is locally known as ‘Karvi’. It undergoes gregarious blooming every 7-10 years during the monsoon months (June-September), a phenomenon that blankets entire hillsides in hues of violet and white [3]. Previous studies have documented occurrence of S. callosus in several locations within the Western Ghat regions of Maharashtra such as Ahmednagar, Kolhapur, Pune, Raigad, Ratnagiri, Satara, Sindhudurg, and Thane. [42] [Fig.1A]. Ecological significance: The mass flowering of S. callosus is a strategy that attracts a wide spectrum of pollinators- bees, butterflies, birds, enhancing the cross-pollination and reproductive success [15,16]. The blooms are playing crucial ecological roles such as enriching biodiversity through pollinator support, contributing to the forest floor nutrient cycle through post-bloom decomposition, aiding in soil stabilization and groundwater retention in semi-evergreen and deciduous ecosystems [39]. The life cycle and bloom rhythm of S. callosus not only mark a striking natural spectacle, but also underpin essential ecological processes in the Western Ghats. [20, 30]. Ethno medicinal role: The genus Strobilanthes contains more than 209 bioactive compounds, including phenolics, flavonoids, and terpenoids, many with antibacterial and anti-inflammatory properties [7,47,48]. Traditionally, S. callosus has been used for treating inflammation and arthritis [19,37]. The stems are employed for hut construction and fencing by tribal communities, and the mass blooming supports the production of ‘Karvi honey’, highly valued for its unique taste and medicinal value [6,28]. In some parts of India; the roots are used as medicine and the locals employ this plant for their habitat, constructing walls for roofing, cow enclosures etc. [5]. GIS-based studies have focused on other species such as S. kunthiana and S. blume, highlighting the research gap for S. callosus [17]. Despite its ecological and cultural importance, detailed floristic and reproductive studies on S. callosus, particularly in Maharashtra, remain scarce. While prior studies have addressed broader ecological roles and pharmacology of Strobilanthes species, particularly in terms of a few species like S. kunthiana, S. blume, S. ixiocephala; there is a need to dearth systematic data on the floral biology of S. callosus including morphometrics of flowers, pollen and fruit characteristics, and reproductive efficiency of this underutilized plant species. MATERIALS & METHODS Study Area Strobilanthes callosus is predominantly distributed across the Western Ghats of India, specifically in the states of Maharashtra, Karnataka, and Tamil Nadu. The present study was conducted in selected habitats of S. callosus within the Nashik district of Maharashtra, primarily at the Trimbakeshwar and Anjaneri hills, which are known for their rich floristic diversity and periodic blooming of Strobilanthes callosus [Fig. 1 A-B]. Field Sampling Field investigations were carried out during the flowering season from August to December 2024. Plant specimens were collected and identified using standard regional floras. [8,11.] Weekly field visits were undertaken to monitor flowering phenology, which included the onset of leaf fall, bud initiation, anthesis, end of flowering, fruit development, and seed dispersal stages. At each study site, 25 flowers from 10 randomly selected inflorescences were studied. Observations were first made in the field using a 60× handheld lens, and flowers were subsequently preserved in 70% ethanol for detailed laboratory analysis. Floral visitors were documented through direct observation during daylight hours, particularly between 07:00 and 11:00 hours, when pollinator activity was highest. Visitors were photographed and, when necessary, captured using insect nets for identification with the assistance of entomological keys and expert consultation. Laboratory Analysis Morphometric analyses of floral parts including bracts, petals, stamens, styles, stigmas, ovaries, fruits, and seeds were conducted using digital calipers and a stereo microscope. Phenological stages such as leaf senescence, bud initiation, anthesis, and fruiting were recorded systematically. Anther dehiscence was assessed by examining unopened flowers under a dissecting microscope to determine the proportion of dehisced anthers at anthesis. Pollen grains were collected from freshly dehisced anthers and analyzed for size, shape, and aperture characteristics. Pollen viability was evaluated using the acetocarmine staining method at room temperature, following protocols [34]. In vitro pollen germination tests were performed employing both hanging drop and sitting drop methods, as described by [32,33]. Germination success and viability were documented microscopically and photographed for reference [Fig. 5 c-e]. Data Analysis: Observed data was analyzed using relevant statistical tools to ensure accuracy and reliability. The blooming and anther dehiscence data were analyzed using one-way ANOVA that revealed significant differences in the number of flowers opened and percentage of anther dehiscence across different time intervals. [Table 1]. Similarly, for morphological parameters and pollen viability data [Table 2 and Table 4], One-Way ANOVA was applied to compare plant height, internodes length, and leaf dimensions and assess pollen viability data across the sample plants. RESULTS Strobilanthes callosus is a glabrate shrub with stiff, rough, and warted stems that grows 2-5 meters high. The leaves are 10-22 X 3-7.5, with one pair frequently smaller than the other. They are elliptic-lanceolate, acute or acuminate, with crenate and ciliate margins. [Table 2]. Phenological Observations The flowering phenology of Strobilanthes callosus was monitored from August to December 2024. Leaf senescence began in mid-June, followed by new leaf initiation in early July. Bud emergence occurred during the last week of July, with full flowering observed between mid-August and late September. Anthesis occurred between 7:00 a.m. and 9:30 a.m., while anther dehiscence was recorded within one hour of anthesis. Flowering … Read more

Does leaf turgor loss point (πtlp) differ between nitrogen fixers and non-nitrogen fixers? – A short research

Rincy Antony

Introduction: Nitrogen is a requisite element for plant growth and development as it is responsible for the production of amino acids, which are the building blocks of protein. Additionally, it is an essential component in the synthesis of nucleic acid (DNA and RNA), essential for all living organisms (1). Research studies have shown that the efficient utilization of nitrogen by plants is responsible for an increased root biomass in plants along with a widespread network of roots for better water absorption (2). Although nitrogen makes up 78% of the atmosphere and 98% of the soil as organic nitrogen, plants cannot directly utilize it. Instead, nitrogen must be fixed either through fertilizer production or by microorganisms that form symbiotic relationships with plants (3). This study examines two plant groups—nitrogen fixers and non-nitrogen fixers—to investigate how nitrogen fixation influences turgor loss point (TLP) in a plant. The findings aim to reveal whether nitrogen fixation contributes to better plant survival under drought conditions. The nitrogen-fixing group of plants include Caesalpinia pulcherrima, a common ornamental and medicinal plant in India, belonging to the Caesalpiniaceae family (4,5) . Pongamia pinnata, a nitrogen-fixing tree from the Fabaceae family https://winrock.org/pongamia-pinnata-a-nitrogen-fixing-tree-for-oilseed (6). Albizia saman, belonging to the Leguminosae family, forms nitrogen fixing symbiosis with many strains of Rhizobium, and readily fixes nitrogen by forming root nodules (7). Albizia lebbeck, another species of genus Albizia which possesses nitrogen fixing properties (8) . Lastly, Saraca asoca, a nitrogen fixer that belongs to family Fabaceae and has long existed as a part of Indian traditional medicine, specifically to treat gynecological disorders (9) . The non-nitrogen fixing group of plants include Terminalia arjuna, commonly known as arjuna tree, which belongs to the Combretaceae family (10) . Terminalia bellirica, another plant species of the same family (11). Butea monosperma, a Fabaceae family member (12). Wrightia tinctoria, a non-nitrogen fixer, which belongs to the family Apocynaceae (13) . The last non-nitrogen fixer of this group is Azadirachta indica commonly known as neem which occupies a significant position in Indian traditional medicine (14) . The trait of leaf turgor loss point (πtlp)discussed in this study reflects a plant’s capacity to maintain turgor pressure during leaf dehydration, and is an important predictor of its response to drought (15). Traditionally, turgor loss point (TLP) was measured using an approach of pressure-volume curve also known as pressure-bomb technique developed by Scholander and colleagues (16) . It deals with theoretical analysis of equilibrium water relations of a twig’s cells taking into consideration the fact that each cell has unique shape, solute concentration, fluid content and mechanical strength given by its cell wall structure and attachment to neighboring cells (17) . Pressure-volume curves summarise leaf level responses to increasing water scarcity (18) . Recent studies have demonstrated that the measurement of TLP can be effectively achieved using vapor pressure osmometers and psychrometers (19,21). Studies suggest that plants with more negative TLP can better resist the dehydration of leaves, which in turn helps them to sustain physiological processes like stomatal conductance, photosynthesis and growth even under scarcity of water (22–28) . This research study mainly relies on the leaf TLP estimation of nitrogen fixers and non-nitrogen fixers for the estimation of better drought tolerance. This study quantified differences in leaf turgor loss points (πtlp)between two plant groups i.e., nitrogen fixers and non-nitrogen fixers to assess which plant group has a better tolerance under drought conditions. Materials and Methods: Sample Collection: The plants of both the groups (nitrogen fixers and non-nitrogen fixers) were collected from the National Centre for Biological Sciences (NCBS), Bengaluru, and the Gandhi Krishi Vigyana Kendra (GKVK), Bengaluru, which are specifically planted for research purposes. Sample preparation: The leaf samples for the estimation of TLP were collected one day before and stored in a beaker with proper labeling indicating the date of collection, name of the person who collected the sample, plant species name, and replica number (abbreviated as ‘R’). Five different replicates (R1, R2, R3, R4 and R5) for each plant species were sampled. The leaf samples were cut from the plants by snipping the leaf sheath and making a base cut underwater, taken in a beaker, and were kept submerged to avoid cavitation. Then, the beakers with the leaf samples were kept inside a zip lock bag with moist tissue to ensure that the air inside the bag remained humid. These samples were stored in a dark place and were allowed to rehydrate for about 20-24 hours and were only taken out during TLP estimation. Materials Required: Psychrometer, tissue roll/ paper towels, aluminum foil, cork borer or puncher, sharp-tipped tweezers/insect pin, liquid nitrogen, protective gloves, forceps/ tongs, pipette tip of any size, leaf samples (rehydrated for 20-24 hours). Osmotic Pressure (πosm) estimation using Psychrometer: On the day of measurement the psychrometer was allowed to equilibrate for 20-30 minutes after applying grease on the outer corners of the measurement chamber. The chamber was carefully closed and the lid was tightly secured with the help of masking tape. After equilibration the prepared leaf samples were taken out and gently wiped using tissue paper, to ensure no water content was present on the leaf surface at the time of measurement. After patting dry the leaf, it was gently rubbed with sandpaper to remove any trichomes present on the surface. Then the leaf was quickly punched using a paper puncture and was covered with aluminum foil within 30 seconds. The covered leaf disc was frozen in liquid nitrogen for about 2 minutes. After 2 minutes, the leaf disc was taken out, carefully opened and then poked 15-20 times with the help of an insect pin. The leaf disc was then carefully placed in the measuring chamber and the chamber was sealed again using masking tape. The first reading was taken after 2 minutes in the ICT software and the rest of the readings were taken with an interval of 10 minutes until stabilized readings were observed. After taking the readings the measuring chamber was carefully cleaned using distilled water and … Read more

Flavonoids in Malpighiaceae: A Comparative Study of Four Species from the Northern Minas Gerais Cerrado

Pedro Henrique Fonseca Veloso¹
Luccas Roberto Pereira Mendes²
Vanessa de Andrade Royo^1,2

INTRODUCTION Malpighiaceae is a botanical family currently comprising 77 genera and approximately 1,350 species distributed across tropical and subtropical regions of the world [1]. In Brazil, 46 genera are found, with a significant concentration of species diversity in the cerrado biome. Notable genera occurring in this biome include Byrsonima, Banisteriopsis, Diplopterys, Heteropterys, Malpighia, and Stigmaphyllon [2,3]. Certain species have been the focus of phytochemical investigations, such as Diplopterys pubipetala, which has been reported to contain flavonoids with antifungal activity, including high efficacy against yeasts of the genus Candida [4]. Studies conducted on Banisteriopsis species from the cerrado have revealed a chemical profile rich in flavonoids [5], suggesting that species and genera within this family may serve as sources of such metabolites and exhibit correlated chemical profiles. Beyond taxonomic classification, the occurrence of sympatric species—particularly congeners sharing the same habitat or ecosystem—raises questions regarding their metabolic richness. This is especially relevant in the context of recent taxonomic reclassifications, such as the division of the genus Banisteriopsis into three genera: Diplopterys, Bronwenia, and Banisteriopsis [6], with reported distributions across different geographic regions [7,8]. With regard to metabolite composition, both Banisteriopsis and Diplopterys have been studied for their phenolic content, including glycosylated flavonoids and aglycones [4,9,10]. In relation to other studies in species of the same family, metabolomic and chemical profiling assays have already been performed [11,12]. Therefore, the aim of this study is to evaluate the phytochemical characteristics of four Malpighiaceae species occurring in a cerrado fragment in northern Minas Gerais, Brazil, which share taxonomic traits, such as yellow flowers. MATERIALS AND METHODS Plant Material Leaves of Banisteriopsis gardneriana (Bg), Banisteriopsis cf. anisandra (Ba), Diplopterys lutea (Dl), and Diplopterys pubipetala (Dp) were collected in the district of Nova Esperança, municipality of Montes Claros, Minas Gerais, Brazil, in February 2025 (coordinates: 16W 33′ 41″, 43S 55′ 30″). Voucher specimens of each species were deposited at the Herbário Norte Mineiro – UFMG under the following voucher numbers: 6223 (Bg), 6225 (Ba), 6222 (Dl), and 6221 (Dp). The samples were registered in the National System for the Management of Genetic Heritage and Associated Traditional Knowledge (SisGen) under registration number A7ED1E1. Extract Preparation The leaves were washed with running water and dried in a forced-air oven (insert brand/model) at 40 °C ± 2 °C for five days. The dried material was ground using an IKA A11 analytical mill (IKA, Germany), and the resulting powder was stored in paper bags inside a freezer (Consul, Brazil) at a temperature between 0 and 4 °C. The hydroethanolic extract (70:30, v/v) was prepared using the exhaustive maceration method, at a ratio of 1 g of plant material to 10 mL of solvent, for seven days in the dark with occasional agitation. The material was filtered, and the solid plant residue was re-extracted with the same volume of solvent. After a second filtration, the combined extracts were concentrated under reduced pressure in a rotary evaporator (SP Labor, Brazil) at 40 °C ± 2 °C. The extraction yields for Bg, Ba, Dl, and Dp were 14.7% (1,470 mg), 16% (1,600 mg), 19.7% (1,970 mg), and 7.1% (710 mg), respectively. Thin-Layer Chromatography (TLC) TLC was performed on aluminum plates precoated with silica gel 60 (0.2 mm thickness) containing a fluorescent indicator F254 (Macherey-Nagel, Düren, Germany). The mobile phase specific for flavonoids consisted of ethyl acetate: glacial acetic acid: formic acid: and water (100:11:11:26, v/v/v/v). Detection was carried out using UV light at wavelengths of 254 nm and 365–395 nm, and chemically by spraying with 5% aluminum chloride (AlCl₃) solution. Rutin and quercetin were used as flavonoid standards. The alignment of solvent front distances for each sample was performed using the dimension tool in CorelDRAW (2021). RESULTS AND DISCUSSION The results obtained under UV light at 254 nm revealed compounds with corresponding retention factors (Rf) shared among some species. B. gardneriana (Bg), D. lutea (Dl), and B. cf. anisandra (Ba) exhibited shared Rf values of 0.70; Bg, D. pubipetala (Dp), and Ba showed Rf values of 0.81; and Bg and Ba shared an Rf of 0.89. Under UV light at 365–395 nm, Bg, Dl, Dp, and Ba shared Rf values of 0.60 and 0.64. After spraying with AlCl₃ and subsequent observation under UV 365–395 nm, Rf values of 0.75 and 0.64 were observed across all species. These Rf values may be closely associated with flavonoids common to the species, or to the family or genus level; given their taxonomic proximity, Diplopterys and Banisteriopsis may contain similar compounds. The retention factors are detailed in Table 1. Rf values corresponding to rutin were observed in three samples: B. gardneriana (Bg), B. cf. anisandra (Ba), and D. lutea (Dl), while quercetin was detected in Bg and Ba. A study on TLC of flavonoids [13], using the same mobile phase as in the present work, indicates typical flavonoid Rf regions as follows: 0.05–0.3 (flavonoid oligosides), 0.25 (flavonol triosides), 0.40 (rutin, quercetin-, kaempferol-, isorhamnetin-3-O-(2’’–6’’-di-O-α-L-rhamnopyranosyl)-β-D-glucopyranoside), 0.45 (narcissin, isorhamnetin-rutinoside, iso-orientin, isovitexin, vitexin), 0.5 (6-hydroxykynurenic acid, kaempferol-, quercetin-3-O-(6’’-trans-p-coumaroyl-4’’-glucosyl)-rhamnoside), 0.6 (quercitrin), 0.75 (isoquercitrin, astragalin, dihydrokaempferol-7-O-glucoside), and front (flavonol aglycones, biflavonoids). These data corroborate the results obtained here, with well-defined zones corresponding to possible flavonoid groups. Regarding Diplopterys pubipetala, previous investigations reported the presence of orientin and vitexin with an Rf of 0.49 [4]. Geographic aspects may directly influence the ecological and ecophysiological interaction networks of the studied flora. The use of aluminum chloride favors the formation of fluorescent complexes in flavonoids, allowing visualization under UVA light, as occurred in the present study [14,15]. which indicates not only the presence but also the diversity of flavonoids in the samples analyzed. Other Rf values were observed sporadically, showing zones of higher and lower intensity, which may relate to the chemical identity of each analyzed species. Thus, the findings indicate not only chemotaxonomic proximity but also the individual chromatographic profile of each species and the richness of flavonoids present in the samples. This may suggest a chemical identity or even physiological and phenotypic plasticity of the species in response to their environmental conditions. CONCLUSION The results obtained in this study highlight the presence of flavonoids in the four species … Read more

Effects of ethanol extract of Ocimum sanctum leave on glycemic, lipidemic, and platelet aggregation status in neonatal streptozotocin-induced type 2 diabetic rats

J. M. A. Hannan
Asma Ahammed
P. R. Flatt

Diabetes mellitus is recognized as a significant health concern worldwide and is a primary determinant of disability, morbidity, and mortality. Nevertheless, progress in knowledge and therapeutic approaches presents significant challenges in managing diabetes [1] [2]. Type 2 diabetes is a widespread and critical chronic condition arising from complex genetic-environment interactions, aggravated by additional risk factors such as obesity and a sedentary lifestyle [3]. There are several different classes of drugs, both oral and injectable, for the management of type 2 diabetes (T2DM) [4]. However, these nonprescription drugs are costly, not freely available to some populations, and often associated with significant drawbacks, let alone the inability to insure against complications of the disease [5]. The World Health Organization (WHO) has documented approximately 21,000 plant species globally that are frequently used for medicinal purposes. Of these, around 2,500 species are utilized in India, with nearly 150 species being commercially available on a large scale. India is recognized as the largest producer of medicinal herbs and is often referred to as the “botanical garden of the world” [6]. According to a WHO survey, traditional medicine systems account for approximately 80% of healthcare treatments in India, 85% in Myanmar (Burma), and 90% in Bangladesh [7]. Medicinal plants play a significant role in the traditional management of type 2 diabetes mellitus (T2DM) across various countries. Many of these plants have demonstrated notable antidiabetic activity, often with minimal or no side effects. These plants are rich sources of bioactive compounds such as flavonoids, alkaloids, phenolics, and tannins, which are known to enhance pancreatic islet function, stimulate insulin secretion or improve insulin action, and reduce intestinal glucose absorption [8]. Due to these properties, herbal medicines have gained widespread popularity, especially in communities with limited access to conventional pharmaceuticals [5]. Such plant-based therapies are often preferred in economically disadvantaged populations due to their affordability, accessibility, and lower risk of drug-related adverse effects. Consequently, they are increasingly being used as alternatives or adjuncts to synthetic antidiabetic drugs to help manage and reduce the complications associated with diabetes [9]. Several studies have demonstrated the antihyperglycemic benefits of medicinal plants in the management of type 2 diabetes mellitus (T2DM) [10]. One prominent example is Ocimum sanctum L. (syn. Ocimum tenuiflorum L.), commonly known as Holy Basil. This plant holds a distinguished place in traditional and folk medicine systems across Southeast Asia [11]. The leaves of O. sanctum have been widely used in Ayurvedic and Unani medicine for their therapeutic properties and have been traditionally applied in the treatment of various ailments, including diabetes, ulcers, and cancers. Additionally, the plant has been used to manage malaria, diarrhea, dysentery, skin diseases, arthritis, eye infections, insect bites, as well as bacterial and fungal infections [12]. Ocimum sanctum contains a complex profile of chemical constituents, including a variety of nutrients and bioactive compounds that contribute to its pharmacological properties [13]. However, the concentrations and efficacy of these bioactive ingredients can vary significantly depending on cultivation practices, harvesting time, processing techniques, and storage conditions [14]. Various parts of the plant—including leaves, stems, flowers, roots, seeds, and even the whole plant—have been employed in traditional medicine for their therapeutic potential. Ocimum sanctum leaves have been traditionally utilized in the management of diabetes mellitus for many years. A dietary supplement of fresh leaves at a dosage of 2 g/kg body weight administered to albino rabbits for 30 days resulted in a significant reduction in blood glucose levels [15]. Additionally, tulsi leaf powder has been reported to significantly decrease serum and tissue lipid profiles in both normal and diabetic rats [16]. Oral administration of the extract led to a marked reduction in blood glucose levels in glucose-fed hyperglycemic and streptozotocin-induced diabetic rats [17]. Ethanolic extracts of O. sanctum also significantly reduced glycosylated hemoglobin and urea levels in streptozotocin-induced diabetic rats, while concurrently increasing levels of glycogen, hemoglobin, and total protein. These changes were accompanied by an increase in insulin levels and improved glucose tolerance [18]. It is believed that several bioactive compounds present in O. sanctum leaf extracts exert stimulatory effects on insulin secretion, thereby contributing to its antidiabetic properties [19]. Supporting this, research by Chandra et al. [20] demonstrated that oral treatment with O. sanctum extract at a dose of 500 mg/kg body weight significantly reduced blood glucose levels in insulin-deficient, streptozotocin-induced diabetic rats. The extract also inhibited lipid peroxidation, reactivated key antioxidant enzymes, and helped restore glutathione (GSH) and antioxidant metal levels. Furthermore, it induced a notable increase in liver glycogen content in both normal and alloxan-induced diabetic rats. To further understand the multiple actions of Ocimum sanctum in type 2 diabetes, the present study aimed to investigate the chronic effects of an ethanol leaf extract on the glycemic, lipidemic, and platelet aggregation status in type 2 diabetic rats. Dried Ocimum sanctum leaves were procured from Ramkrishna Mission, Kolkata, India, and botanically authenticated, with voucher specimens deposited at the National Herbarium, Bangladesh. The leaves were first washed thoroughly with water, and petioles and stems were removed. The cleaned leaves were then oven-dried at 40°C, ground into fine powder (200 mesh) using a Cyclotec grinding machine, and stored in an airtight plastic container. A total of 2 kg of the powdered O. sanctum leaves was subjected to extraction with 80% ethanol (10 L) in a stainless steel extraction tank over four days at room temperature, with the solvent being replaced daily. The combined extracts were filtered and concentrated to dryness using a rotary evaporator. Residual solvent was removed using a membrane pump. The final extract (275 g) was freeze-dried using a Varian 801-model LY-3-TT freeze-dryer (USA) and stored in a reagent bottle at 4°C in a freezer until further use. Adult male Long-Evans rats (180–220 g body weight) were used in all experiments. The animals were housed under controlled environmental conditions at the animal facility of the Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM), Dhaka, Bangladesh. They were maintained on a 12-hour light/dark cycle at a temperature of 21 ± 2 °C. The standard … Read more

Physicochemical and Microbial Properties of eroded coastal soil in Eagle Island Rivers State, Nigeria

Gold Ejire
Aroloye O. Numbere
Peace K. Ohia

Tidal erosion has negative effect in many countries worldwide, including Nigeria. An extremely braided river erodes banks in its entire course by increasing its area and forming and destroying sand bars or chars, causing vast amounts of sediment. At present, tidal erosion is the major natural hazard for the riverside people, which has attracted attention from countries globally [1]. Tidal erosion causes multidimensional, ecological and environmental impacts Tidal erosion leads to the loss of crops, fertile land and other land-based resources. In addition, flooding displaces microbes and destroys the physico-chemical properties of the soil, which in turn is inimical to agricultural growth. [2]. The physicochemical properties and the structure and composition of the soil play key role in preventing tidal erosion. Soil chemistry influences the activities of microorganisms, which in turn play vital role in soil fertility and agricultural development in an agrarian society [3]. Similarly, the proliferation of microorganisms is dependent on the bulk density and soil porosity which is destroyed by erosion [4]. Increase in microbial activities helps in the sustenance of the soil and ensures an increase in productivity. Soil that is reinforced with rich humus soil and roots materials can withstand wind and water erosions [5]. Compaction negatively affect the function and structure of the soil and destroy the microbial population and prevent nutrient flow from the soil to roots of plants [6]. It is thus, important to determine the physico-chemical properties of the soil so as to know the impact of tidal erosion [7]. Tidal erosion has colossal impact on soil fertility and the microbial population [8], but not much have been studied in the Niger Delta region. The ecosystem comprises of the biotic system, which is made up of plants animals and microbes, but not much emphasis is given to the microbial community, which has made this work more necessary. The microbiota are the engine room of the coastal environment because of the increase in organic waste, which has necessitated the actions of decomposers. The trophic dynamic of the coastal community is regulated by the activities of decomposers which drives the energy within and outside the coastal areas. Stochastic changes in the coastal environment increases the unpredictability of natural events, which are worsened by adverse anthropogenic activities [9]. Microbes play role in gaseous exchange and nutrient cycle in terrestrial, aquatic and atmospheric environments [9]. The mangrove forest and its adjoining water bodies provide a conducive environment for microbial activity, which helps the circulation of nutrients [10] in a web of trophic activities. Since mangrove forest is a haven for multiple species decomposition activity is vital source of energy. Therefore, the washing away of the soil creates a dislocation that destabilizes ecosystem function. The soil physico-chemical property facilitates or impedes the biotic processes of the soil. Litter fall from mangrove trees provide the raw materials for decomposers to break down organic materials to fertilize the soil. Coastal erosion can also add or remove nutrients from the soil Tidal erosion, which is the wearing a way of coastal soil [12] is a problem in many coastal communities because of human-mediated activities of deforestation and sand removal for piling buildings constructed on wetlands. Locals dig up chikoko soil and use it for reinforcing the building they erect on the swamp. Scooping up soil fragments the soil and makes it vulnerable to tidal erosion, which further accretes the soil and wash away microbes and other soil-dwelling organism. In the same vein, the number of trees in mangroves forest has declined drastically over the years because of firewood production and urban renewal project for the construction of houses. Flooding from high volume of water from terrestrial area add to the erosional process by creating a wetland depleted of nutrients and microbes. The shape of the land also add to the severity of the flooding, sloppy areas leads to high acceleration of run-off water which washes the soil and deposits it in the river as sediments. The soft nature of the soil makes it wash faster compared to rocky soil. The Niger Delta soil is derived from sedimentary rock that is a soft alluvial thus, it erodes easily During the rainfall season erosion intensifies because the accumulation of upland water drains into the river [13] while during high tide the river water overflows and washes away the edges of the shore line. The topography of the study area Eagle Island, which has low elevation help to accelerate erosional processes. But before the removal of the forest the mangrove trees help to break and stop the erosion force and reduce the speed of the incoming water. While the tree roots hold the soil together to prevent both wind and water erosion from washing the soil particles. . The microbial community make up the decomposers, which in turn include fungi, protest, bacteria and earthworms. At the study site the fungal group include molds and mushrooms and they grow and break down the remains of dead plant and animal matter. On the other hand decomposers are microscopic decomposers that cause decay [14]. Bacteria and fungi break down dead organic matter in the soil. Ground dwelling organisms such as earthworms and fiddler crabs (Uca tenageri) bore holes and create burrows. Which help to aerate the soil and increases its fertility for plant growth [15], which has made the wetland to be a biodiversity hotspot [15]. But erosion destroys and wipes out the organisms and removes their ecosystem services. The breaking away of the soil hill by tidal force creates a sandy coastal plan whose physicochemistry is different from the usual muddy soil known as “chikoko”[16]. The nature of the soil and water content determine the extent of erosion. Lose soil will move faster and easily carried by tidal force. The river picks up and move particles and pebbles along the way, which causes abrasion of the river bottom [17] leading to sediment formation that smother benthic and terrestrial organisms. The destabilization of the pedology leads to the dysfunction in the productivity … Read more

Lawsonia inermis L. (Henna): A Comprehensive Review of Its Phytochemistry, Pharmacological Potential, Traditional Uses, and Commercial Applications

Chetan Joshi
Kavan Shukla
Kunal N. Odedra
B. A. Jadeja

1. Introduction Lawsonia inermis L., commonly referred to as henna, is a perennial shrub belonging to the family Lythraceae, which has a long history of use in traditional medicine and cultural rituals. The plant is particularly famous for yielding a reddish-orange colour from the compound lawsone (2-hydroxy-1,4-naphthoquinone), which is found primarily in its leaves. Lawsone, as reported by Oda et al. (2018), is the major active compound that gives henna its colouring properties, which have contributed to its being a standard ingredient in cosmetic and ceremonial applications for centuries. [49] highlighted the broad geographical and cultural significance of L. inermis in Asia, Africa, and the Middle East, where it finds application in rituals, healing, and as a plant dye. Apart from its colouring role, the plant contains a wide variety of phytochemicals—e.g., triterpenoids, flavonoids, and phenolic acids—which serve to promote a wide variety of biological activities. [9] has named these compounds as being responsible for their drug efficacy. Traditionally, L. inermis‘ therapeutic uses have been recognised by traditional medical systems worldwide. It was reported used by [35] in Ayurvedic medicine, and [20] used it in wound care, infection management, and metabolism disorder treatment via generation-to-generation transmission of ethnomedicinal information. These conventional claims have been provided with scientific justification by recent experimental research. Its antioxidant, antidiabetic, antimicrobial, and anti-inflammatory activities were documented by [42], [50], and [2] further investigated its immunomodulatory and wound-healing activities, further establishing its significance in current phytotherapeutic research. Notwithstanding the growing amount of evidence, hurdles like extract standardisation, toxicity testing, and strict clinical trials are still present. The review herein covers an overall discussion on L. inermis, including its taxonomy, phytochemistry, ethnopharmacological history, therapeutic attributes, and prospects in commercialisation. This is aimed at bridging traditional knowledge with contemporary science to aid future innovation and sustainable use of this multipurpose plant. 2. Taxonomy and Botanical Description L. inermis L. is the single known species in the genus Lawsonia and is found in the family Lythraceae, which is placed in the order Myrtales. Its taxonomic status as a dicotyledonous angiosperm has been repeatedly upheld in every classification scheme that has been put forth by Bentham and Hooker, Engler and Prantl, Cronquist, and the Angiosperm Phylogeny Group (APG). [49] had characterised L. inermis as a much-branched, smooth shrub or small tree, usually 2 to 6 metres tall. The plant is readily identified by its opposite, simple leaves that range from elliptic to lance-shaped shapes, usually 1.5 to 5 centimetres long [4,6]. These leaves have entire margins and acute tips and are either sessile or attached by very short petioles. The plant bears fragrant flowers arranged in terminal panicles. Each flower consists of four petals, from white to pale pink, and has prominent stamens. [9] reports that the fruits are tiny, spherical capsules measuring 4–8 millimetres in diameter, containing numerous angular seeds. The bark is typically thin and greyish-brown, and it peels in fine layers as the plant grows [7,10]. One of the major botanical features of L. inermis is that it produces the orange-red pigment lawsone, which is deposited in the leaves [49]. observed that the pigment is significant from a historical point of view for its applications in traditional body adornment, natural hair dyeing, and colouring of fabrics. Table 1. Comparative taxonomic classification of L. inermis L. according to different botanical systems (Bentham & Hooker, Engler & Prantl, Cronquist, and the Angiosperm Phylogeny Group). Figure I. This figure details the morphological features of L. inermis (henna). (a) presents the mature plant naturally occurring in its environment, with its bushy appearance- (b) provides a flowered branch, which emphasises the opposite phyllotaxy also seen here clearly in the inset. (c) offers a single flowered twig with minute, opposite leaves. (d) gives a close-up of the leaves, which are elliptic, entire, and oppositely arranged on the stem. (e) gives the inflorescence with many minute flowers aggregated in terminal cymes. (t) gives a close-up of one flower, displaying whitish-yellowish petals and bold stamens. (g) gives a single stamen with a bilobed anther, and (h) gives a mature green fruit, which is a tiny capsule. 3. Synonyms and Vernacular Names The botanical name Lawsonia was given in commemoration of Dr. Isaac Lawson, an 18th-century Scottish doctor. The species name inermis refers to the usually spineless twigs of the plant, which separate it from other thorny shrubs. Because of its wide geographic range and morphological variability, L. inermis has been placed under different synonyms in the past. These synonyms, documented in ancient botanical works, are listed in Table 2. L. inermis has a large number of vernacular names, which highlight its extensive cultural and medicinal use. It is “henna” or “Egyptian privet” in the English language [1]. It is named as “mehndi” in Urdu and Hindi, “mendi” in Gujarati and Marathi, and “maruthani” in Tamil. In the Arabic-speaking world, it is also known as “hina” or “henné.” There are similar names given to it in Persian and Swahili cultures. As identified by [1], an array of vernacular names is employed throughout Asia and Africa, each signifying the plant’s function in religious, cosmetic, and medicinal practices. Some examples include “inai” in Indonesian and Malay, “dan” in Burmese, “kaaw” in Lao, and “thian daeng” in Thai. Table 3 presents a list of these names to show the local vocabulary and cultural extent of L. inermis. 4. Cultivation and Distribution L. inermis, or henna, is a hardy plant species that withstands dryness and semi-desert conditions. The plant tends to grow up to a size of about six metres but can be kept in check at a shorter height by way of pruning, thereby maximising production of leaves as a harvest commodity, as has been observed by [50]. Ideal growth is achieved in sandy, drained soils under full sun and is particularly well-suited for tropical and subtropical environments. It is restricted in the colder areas by its frost sensitivity [50]. In the conventional farm environments, L. inermis is commonly planted on field borders or around residences and serves … Read more

Enriched compost with vivianite and pyroclastite powders: suitable fertilizer for better maize growth under the High Guinean Savanah climate of Cameroon

Mohamed Awal
Tchuenteu Tatchum Lucien
Simo kuate David
Megueni Clautilde

INTRODUCTION Zea mays L., also known as maize, is a food crop of the grass family. This cereal is one of the most important crops for human consumption, with an annual world production of approximately 2.80 billion tons. It is one of the world’s main cereal crops, and thus a mainstay of global food security [1]. Maize is rich in starch (around 70%), fat, protein, ash, mineral elements (potassium, magnesium, and phosphorus), and crude fiber [2]. Cameroon produced 2 million tons of maize in 2020, ranking 14th in Africa [3]. Despite the high level of maize production, requirements are constantly increasing because of the combined effects of rising human and animal consumption. Cameroon is both a maize importer and exporter, depending on the season. In seasonal abundance times (October to mid-January), the maize prices on local markets are low, and Cameroon exports maize to West African markets and even to Europe. When stocks run out from January to February onwards, prices on local markets generally rise until September. The price relationship with the international market then reverses, then Cameroon increases its maize imports [4]. Maize production in Africa increases, and in general, there is still great potential to increase productivity. However, its production is limited in the Adamawa-Cameroon region by many abiotic and biotic constraints that affect the yields. These include soil poverty. In this respect, maize growers in the Adamawa-Cameroon region generally use chemical fertilizers to solve the problem of soil deficiency in mineral elements, in order to optimize the yield of this cereal crop. However, several authors [5] [6] revealed that chemical fertilizers have an immediate positive effect on plant growth potential, but present serious environmental risks and do not maintain soil fertility. In this respect, it is urgent to consider management methods in local farming that can increase agricultural production while protecting the environment. Growing maize using compost combined with rock powders would contribute to improving the seed yield, cleaning up the environment, as well as adding value to our local material in agriculture while protecting the environment. Compost has physicochemical and biological properties that improve soil structure [7] [8]. Compost is an organic amendment that improves soil biodiversity through the contribution of microorganisms, combats mineral depletion, improves physicochemical qualities, and helps reduce the need for industrial nitrogen fertilizers [9]. The Adamawa region is rich in rock deposits, including vivianite in the Hangloa village and basaltic pyroclastites around Lac Tison. Vivianite is rich in phosphorus, then it can be used to improve soil fertility, consequently enhancing plant growth and yields [10]. Pyroclastites are characterized by their mineralogical composition rich in exchangeable bases (Ca2+, Mg2+, and K+) that can improve soil chemical properties [11]. This study aims to improve the maize productivity under the High Guinean Savannah climate of Adamawa-Cameroon without using chemical fertilizers. Specifically, it consists to: (1) evaluate growth substrates (soil, composts) for maize growth; (2) determinate determining the effects of the combination of composts and rock powders on maize production; (3) estimate the economic value of fertilizers. MATERIALS AND METHODS Study site The study was conducted during two cropping seasons (2023 and 2024) in the experimental field of the University of Ngaoundere (Cameroon) located at Darang locality. The area belongs to the agroecological zone II of Cameroon and is characterized by a Sudano-Guinean Savannah with six month’s dry season (November to April) and six months rainy season (May to October). The Adamawa Regional (Cameroon) Meteorological Service provided the meteorological data (precipitation and temperature) for both cropping years. Precipitation is higher in 2023 than in 2024, with an average of 197.11 mm per month and an annual total of 2365.30 mm in 2023, compared with 116.97 mm per month and an annual total of 1403.70 mm in 2024. May and June are the wettest months in 2023, with 415.30 mm and 493.10 mm of precipitation, respectively. In 2024, May and September are the wettest months, with 240.30 mm and 332.20 mm of precipitation, respectively. Average temperatures are higher in 2024 than in 2023, with an average of 24.25°C in 2024 versus 22.10°C in 2023. March and April are the hottest months in 2024, with average temperatures of 29°C and 28°C, respectively. In 2023, March and May are the hottest months, with mean temperatures of 24.20°C and 23.60°C, respectively (figure 1). The vegetation of the area is an herbaceous savannah dominated by Imperata cylindrica, Annona senegalensis, and Piliostigma thonningii. The following are the geographical parameters of the field: 07°23′ latitude North, 13°29′ longitude East, and 1125 m altitude. P: precipitation, T: temperature Material Maize seeds The seeds of the SHABA maize variety are used (Figure 2). These seeds were supplied by the Institute of Agricultural Research for Development of Wakwa (Ngaoundere, Cameroon). This variety was chosen for its great adaptability to the rainy season, early germination, and it has short reproduction cycle. Its development cycle varies between 100 and 120 days. These seeds are white, they are oval-shaped and medium-sized, with an average length of 10.50 mm and an average width of 8.5 mm, average weight is between 250 and 300 mg. Fertilizers The fertilizers (figure 3) used in the trials include: 03 different types of compost (compost derived from poultry litter, cow dung manure, and goat droppings manure), rock powders (pyroclastites and vivianite), and chemical fertilizers (NPK 20-10-10 and Urea 46 % N). Animal wastes (poultry litter, cow dung, and goat droppings) were collected from livestock buildings located near the campus of the University of Ngaoundere. The composting method, according to [5], was used. The composting process lasted 04 months. Vivianite was collected in the Hangloa locality located between 7o20′ and 7o30′ North latitude and 13o20′ and 13o25′ East longitude. The chemical composition of vivianite powder is the following: Fe2O3 (68.72%), P2O5 (9.17%), Al2O3 (7.72%), and SiO2 (9.67%) [10]. Thus, the total phosphorus content was estimated at about 671.50 mg/kg, while the assimilated phosphorus content was around 81.13 mg/kg. Phosphate contained in this mineral can be solubilized. Pyroclastites were collected around Lake Tyson … Read more