Study on biocontrol aspect of potential Alcaligenes faecalis against Fusarium sp.,Concept and Approach

  • Purnima1
  • Pooja Singh2

Purnima1 , Pooja Singh2

Department of Industrial Microbiology, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur- 273009, Uttar Pradesh, India1

Department of Botany, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur- 273009, U.P, India2

Corresponding Author Email: purni9393ma@gmail.com

DOI : https://doi.org/10.51470/JPB.2024.3.1.34

Abstract

The world is dealing with vivid pollutions which lead to the degradation of resources and human health. In soil, the yield of crops is enhanced by the rampant use of chemical pesticides and fertilizers. The fungicides are applied on a large scale on almost all crops to control fungal infection which is the primary cause of crop productivity loss globally. The chemical fungicides are nevertheless causing great harm to soil quality and human health as many fungal pathogens are resistant to these chemical fungicides and all applied in bulk. There is a huge need for the substitution of such fungicides by natural components. The work is just an attempt in this aspect only to glorify Alcaligenes faecalis as a natural substitute for chemical fungicides as its bio- remediating aspect is explored earlier. The least explored association of Alcaligenes faecalis with Fusarium sp. which could be a futuristic fungicide is represented. Fusarium sp. (mainly Fusarium oxysporum and Fusarium incarnatum) are quite pathogenic causing great loss to various plants and crops across the globe. They are altogether responsible for wilt, leaf spot and root rot diseases. Alcaligenes faecalis as a potent biocontrol agent is introduced here as this bacterium has plant growth- promoting features too. So, Alcaligenes faecalis could become a natural, futuristic and biological fungicide and fertilizer. The large-scale bioinoculant production with its specific carrier molecules needs to be explored.

Keywords

Alcaligenes faecalis, antagonistic activity, biocontrol agent, biological fungicides, Fusarium incarnatum, Fusarium oxysporum

Download this article as:

Alcaligenes faecalis: An emerging biocontrol agent

Alcaligenes faecalis was isolated from a wastewater bioprocessor in Texas, Houston, (USA) [1]. This genus is found in water, soil and in humans, generally non-pathogenic in nature. It is Gram-negative, aerobic, motile and rod-shaped which shows reactivation with Tryptone Soya broth (DSMZ medium 220a). It is mesophilic and shows optimum growth at 30-37oC. The genus is urease, gelatinase, arginine dihydrolase, beta-glucosidase negative, and cytochrome oxidase (enzyme activity) positive [2]. It belongs to Domain- Bacteria, Phylum- Pseudomonadota, Class- Betaproteobacteria, Order- Burkholderiales, Family- Alcaligenaceae, Genus- Alcaligenes and Species- faecalis.

Applicability of Alcaligenes faecalis

The bacteria has multitudinal aspects and cause bioremediation of phenanthrene, polyaromatic hydrocarbons, phenols, pesticides, and azo dyes. Alcaligenes faecalis also converts the most toxic form of arsenic, arsenite to a less fatal form, arsenate. It shows tolerance to heavy metals and has nanoparticle production, biocontrol, and nematicidal activity [3]. It also produces detergents, gum, and bioplastics [4]. This genus has high applicability still it remains under-represented and understudied at the whole genomic level [5].

Fusarium species: A virulent phytopathogen

Fusarium belongs to the Phylum Ascomycota, Family Nectriaceae, and has a broad host range. 120 various formae speciales showing specificity to the host species are determined. It causes damage and disease in cotton, date palm, banana, and other field crops including flowers and vegetables. The disease symptoms are distinguishable viz., vascular wilt, leaf epinasty, lower leaf yellowing, stunted growth, defoliation, and plant death eventually. The only measure to control this disease to a certain extent is the application of resistant varieties [6].

Fusarium oxysporum widely occurs among root region microbes. Mostly it is saprophytic but certain species cause root rot and wilting and are pathogenic. A new approach to biocontrol activity is the application of nonpathogenic Fusarium sp. which combats the pathogenic one [7].

Fusarium sp.: A specific study of diseases occurring in plants

The great loss in productivity of tomatoes is caused by wilt disease of Fusarium oxysporum f. sp. lycopersici. Still, no definite treatment for such a disease is reported. The pathogenic form of Fusarium oxysporum causes wilt disease classified under formae speciales depending on its host. The main reason for Fusarium wilt is its introduction in certain places rather than the local eruption of this pathogen. The soil population of the fungi is mainly ruled by asexual reproduction [8].

Musa spp. (Banana) is widely produced and consumed fruit. Panama disease (Fusarium wilt) causes a huge loss in the yield of bananas. The most effective measure to deal with this is again the use of resistant cultivars to a certain limit [9].

Vascular wilt of tomato is caused by Fusarium oxysporum f. sp. lycopersici. Symptoms include dark brown vascular tissues, wilting and apical discoloration leading to plant death. Wilt disease occurs due to mycotoxins, mycelial accumulation around the xylem, tylose production, and host defense inhibition [10]. (Table 1) [18]

A.Wilting of cotton seedlings.

B.Brown staining of vascular tissue.

C.Streak of wilt infection in taproot.

Spinach, radish, lamb’s lettuce, lettuce, wild rocket, and cultivated rocket production are adversely affected by the Fusarium incarnatum equiseti species complex. This leads to leaf spot disease in leafy vegetables [13]. Fusarium incarnatum results in crown rot disease in Cucumis sativus. White mycelia covered the young fruit leading to its withering. The disease symptom was similar to the fruit rot disease of Botrytis cinerea [14]. Postharvest disease, melon rot is caused by the Fusarium incarnatum equiseti species complex [15].

Alcaligenes faecalis as a biocontrol agent

Plant growth-promoting rhizobacteria are a group of beneficial rhizospheric bacteria that enhance plant growth and yield. The PGPR attribute of Alcaligenes faecalis for different crops has been least explored. It was reported by Jia R. et al (2022)[16], that Alcaligenes faecalis Juj 3 upon seed inoculation showed biocontrol activity against Plasmodiophora brassicae in cabbage and Chinese cabbage. It reduced clubroot disease in cabbage (51.4%) and Chinese cabbage (37.7%). It enhanced cabbage chlorophyll content (23.3%) and root length (49%). It also enhanced photosynthesis upon treatment in normal and Plasmidiophora brassicae-stressed environments.

Antagonistic activity of Alcaligenes faecalis against Fusarium oxysporum was reported by Sayyed RZ and Chincholkar SB (2009)[17]. The antifungal activity occurred due to siderophore production by Alcaligenes faecalis. 75 µl siderophore-broth gave the best inhibition of Fusarium oxysporum [17].

Alcaligenes faecalis subsp. faecalis S18 gave the highest suppression of Fusarium wilt of tomato as observed by Abdallah RAB et al. (2016) [18]. This isolate also enhanced plant height, aerial part fresh weight, root length, and fresh weight. The antagonistic activity of the bacteria was due to chitinolytic, pectinolytic and hydrogen cyanide production Table 2 and Fig 2 [18].

Fusarium oxysporum f. sp. cepae growth was inhibited by 67.46% by consortia of Bacillus mycoides and Alcaligenes faecalis [19]. Biocontrol activity of Alcaligenes faecalis N1-4 against Fusarium graminearum and Fusarium equiseti was reported by Gong AD et al. (2019) [20].

Conclusions and Future Perspective

The work sheds light on the preambling to the antagonistic effect of Alcaligenes faecalis. Biocontrol aspect of Alcaligenes faecalis only against a Fusarium species viz, Fusarium oxysporum and Fusarium incarnatum are described as the most virulent species affecting global crops and leading to great economic loss. Emphasis on biocontrol activity (PGPR attribute) of Alcaligenes faecalis is registered here as it is still under-explored. The most problematic content is no fungicides and other specific measures could combat this fungal pathogen.

There is a great demand for such bioinoculants and biological control agents in order to deal with the wilt and rot of Fusarium sp. The product should be cost-effective and eco-friendly and promote sustainable agriculture. The future of this study demands examining Alcaligenes faecalis inoculant against Fusarium sp. under field conditions on different crops and plants.

Conflict of Interest

The author has no conflict of interest in publishing this work.

References

  1. Rehfuss M. and Urban J. 2006. Alcaligenes faecalis subsp. phenolicus DSM 16503. https://www.dsmz.de/collection/catalogue/details/culture/DSM-16503. Accessed on 23rd Dec, 2023.
  2.  Rehfuss M and Urban J. 2013. Alcaligenes faecalis subsp. phenolicus J is an aerobe, mesophilic, motile bacterium that was isolated from wastewater bioprocessor. https://bacdive.dsmz.de/strain/338. Accessed on 23rd Dec, 2023.
  3. Ju S., et al. Alcaligenes faecalis ZD02, a novel nematicidal bacterium with an extracellular serine protease virulence factor. Appl. Environment Microbiology. 2016. 82:2112-2120.
  4. Tripathi AD., Srivastava SK., and Singh RP. Statistical optimization of physical process variables for bioplastics (PHB) production by Alcaligenes sp. Biomass Bioenergy. 2013. 55: 243-250. Doi- 10.1016/j.biombioe.2013.02.017.
  5. Basharat Z., Yasmin A., He T., and Tong Y. Genome sequencing and analysis of Alcaligenes faecalis subsp. phenolicus MB207. Scientific Reports. 2018. 8: 3616. Doi- 10.1038/s41598-018-21919-4
  6. Michielse CB., and Rep M. Pathogen profile update: Fusarium oxysporum. Molecular Plant Pathology. 2009. 10(3): 311.
  7. Fravel D., Olivain C., and Alabouvette C. Fusarium oxysporum and its biocontrol. New Phytologist. 2003. 157(3): 493-502.
  8. Widnyana K., et al. Pseudomonas Alcaligenes, potential antagonist against Fusarium oxysporum f. sp. lycopersicum the cause of Fusarium wilt disease on tomato. Journal of biology, agriculture and healthcare. 2013. 3(7).
  9. Ploetz RC. Fusarium wilt of banana. Phytopathology. 2015. 105(12): 1521-1521.
  10. Srinivas C., et al. Fusarium oxysporum f. sp. lycopersici causal agent of vascular wilt disease of tomato. Biology to diversity. A review. Saudi J. of biological sciences. 2019. 26(7): 1315-1324.
  11. Diaz RMJ., et al. Fusarium wilt of chickpeas: Biology, ecology and management. Crop protection. 2015. 73: 16-27.
  12. Hu J., and Norton R. Fusarium wilt of Cotton. 2020. Extension.arizona.edu/pubs/az1852-2020.pdf. Accessed on 21st June, 2024.
  13. Matic S., et al. Emerging leafy vegetable crop diseases caused by the Fusarium incarnatum-equiseti species complex. Phytopathology Mediterr. 2020. 59: 303-317.
  14. Mao YS., et al. Occurrence of crown rot disease caused by Fusarium incarnatum on Cucumber (Cucumis sativus) in China. Plant disease. 2020. 104 (2): 593.
  15. Lima EN., et al. A novel lineage in the Fusarium incarnatum-equiseti species complex is one of the causal agents of Fusarium rot on melon fruits in Northeast Brazil. Plant Pathology. 2021. 70(1): 133-143.
  16. Jia R., Chen J., Hu L., Liu X., Xiao K., and Wang Y. Alcaligenes faecalis Juj3 alleviates Plasmodiophora brassicae stress to cabbage via promoting growth and inducing resistance. Front. Sustain. Food Syst. 2022. 6. Doi- https://doi.org/10.3389/fsufs.2022.942409
  17. Sayyed RZ., and Chincholkar. Siderophore-Producing Alcaligenes faecalis exhibited more biocontrol potential vis-à-vis chemical fungicide. Current Microbiology. 2009. 58(1): 47-51.
  18. Abdallah RAB., et al. Isolation of endophytic bacteria from Withania somnifera and assessment of their ability to suppress Fusarium wilt disease in tomato and to promote plant growth. J. Plant Pathology Microbiology. 2016. 7(5): 2-11.
  19. Dinata GF., et al. In vitro evaluation of the effect of combined indigenous antagonistic bacteria against Fusarium oxysporum. International J. of Agriculture System. 2023. 11(1): 55-64.
  20. Gong AD., et al. Inhibitory effect of volatiles emitted from Alcaligenes faecalis N1-4 on Aspergillus flavus and Aflatoxins in storage. Frontiers in Microbiology. 2019. 10: 1419.
Post Views: 378