Plant tissue culture has emerged as a crucial technique in modern agriculture, particularly in the realm of micropropagation. This method offers a multitude of advantages, including the rapid production of disease-free and genetically uniform plant material. In the context of banana cultivation, tissue culture techniques have revolutionized the production of high-quality planting material, addressing various challenges faced by traditional propagation methods. This article explores the advancements in plant tissue culture, focusing on its application in banana cultivation. It examines the process of micropropagation, highlighting its role in enhancing the quality and quantity of banana yields. Additionally, the article discusses the significance of genetic uniformity and disease resistance in banana cultivation, both of which are facilitated by tissue culture techniques. Furthermore, it delves into the optimization of culture media and environmental conditions to maximize the efficiency of micropropagation. Through a comprehensive analysis of current research and practices, this article aims to provide insights into the role of plant tissue culture in advancing quality material production in banana cultivation.