The increasing prevalence of antimicrobial resistance and the environmental concerns associated with synthetic antimicrobial agents have stimulated interest in plant-derived bioactive compounds as sustainable alternatives. The present study evaluated the antimicrobial potential of extracts obtained from Trigonella foenum-graecum and Ocimum basilicum through in silico and in vitro analyses. Phytochemical constituents were assessed for their interactions with selected microbial targets, while antimicrobial activity was determined against representative bacterial and fungal pathogens. The extracts exhibited significant inhibitory effects against the tested microorganisms, indicating the presence of biologically active secondary metabolites with antimicrobial properties. Minimum inhibitory concentration (MIC) assays demonstrated a concentration-dependent response, with increased extract concentrations resulting in greater inhibition of microbial growth. The observed antimicrobial activity is likely associated with the synergistic action of phenolic compounds, flavonoids, alkaloids, and other secondary metabolites present in the plant extracts. The findings highlight the potential of T. foenum-graecum and O. basilicum as natural sources of antimicrobial agents and support their possible application in controlling pathogenic microorganisms affecting agriculture and human health.